CRISPR-U™ Gene Knockout Cell Line
Strategy
PTGER3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PTGER3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PTGER3 |
| Gene id | 5733 |
| Organism | Homo sapiens |
| Official full symbol | prostaglandin E receptor 3 |
| Gene type | protein-coding |
| Also known as | EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3-VI, EP3e, PGE2-R, lnc003875 |
| Summary | The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PTGER3-206 | ENST00000370924.4 | 6982 | 388aa | Protein coding | CCDS658 | P43115-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PTGER3-201 | ENST00000306666.9 | 2333 | 390aa | Protein coding | CCDS657 | P43115-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PTGER3-204 | ENST00000356595.8 | 1943 | 418aa | Protein coding | CCDS655 | P43115-5 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PTGER3-207 | ENST00000370931.7 | 1914 | 390aa | Protein coding | CCDS656 | P43115-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PTGER3-212 | ENST00000628037.2 | 1815 | 365aa | Protein coding | CCDS44160 | P43115-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PTGER3-209 | ENST00000460330.5 | 1447 | 374aa | Protein coding | CCDS652 | P43115-4 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PTGER3-208 | ENST00000370932.6 | 1841 | 388aa | Protein coding | - | A0A0A0MRP3 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PTGER3-203 | ENST00000354608.9 | 1470 | 412aa | Protein coding | - | F8WAJ7 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PTGER3-202 | ENST00000351052.9 | 1404 | 390aa | Protein coding | - | P43115-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| PTGER3-211 | ENST00000497146.5 | 1971 | 425aa | Nonsense mediated decay | - | A0A0B4J204 | - | TSL:1, |
| PTGER3-205 | ENST00000361210.6 | 1922 | 402aa | Nonsense mediated decay | - | O00325 | - | TSL:1, |
| PTGER3-210 | ENST00000479353.5 | 1895 | 393aa | Nonsense mediated decay | - | P43115-7 | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PTGER3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PTGER3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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