CRISPR-U™ Gene Knockout Cell Line Strategy

PTGER3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTGER3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTGER3
Gene id 5733
Organism Homo sapiens
Official full symbol prostaglandin E receptor 3
Gene type protein-coding
Also known as EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3-VI, EP3e, PGE2-R, lnc003875
Summary The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTGER3-206 ENST00000370924.4 6982 388aa Protein coding CCDS658 P43115-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PTGER3-201 ENST00000306666.9 2333 390aa Protein coding CCDS657 P43115-1 - TSL:1, GENCODE basic, APPRIS P4,
PTGER3-204 ENST00000356595.8 1943 418aa Protein coding CCDS655 P43115-5 - TSL:1, GENCODE basic, APPRIS ALT2,
PTGER3-207 ENST00000370931.7 1914 390aa Protein coding CCDS656 P43115-1 - TSL:1, GENCODE basic, APPRIS P4,
PTGER3-212 ENST00000628037.2 1815 365aa Protein coding CCDS44160 P43115-3 - TSL:1, GENCODE basic, APPRIS ALT2,
PTGER3-209 ENST00000460330.5 1447 374aa Protein coding CCDS652 P43115-4 - TSL:1, GENCODE basic, APPRIS ALT2,
PTGER3-208 ENST00000370932.6 1841 388aa Protein coding - A0A0A0MRP3 - TSL:5, GENCODE basic, APPRIS ALT2,
PTGER3-203 ENST00000354608.9 1470 412aa Protein coding - F8WAJ7 - TSL:5, GENCODE basic, APPRIS ALT2,
PTGER3-202 ENST00000351052.9 1404 390aa Protein coding - P43115-1 - TSL:5, GENCODE basic, APPRIS P4,
PTGER3-211 ENST00000497146.5 1971 425aa Nonsense mediated decay - A0A0B4J204 - TSL:1,
PTGER3-205 ENST00000361210.6 1922 402aa Nonsense mediated decay - O00325 - TSL:1,
PTGER3-210 ENST00000479353.5 1895 393aa Nonsense mediated decay - P43115-7 - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTGER3
This KO Strategy loading
Red Cotton™ Notes Gene PTGER3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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