CRISPR-U™ Gene Knockout Cell Line Strategy

PTBP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PTBP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PTBP1
Gene id 5725
Organism Homo sapiens
Official full symbol polypyrimidine tract binding protein 1
Gene type protein-coding
Summary This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Genomic regions Chromosome 19
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PTBP1-203 ENST00000356948.11 3206 557aa Protein coding CCDS42456 P26599-3 NM_002819.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PTBP1-204 ENST00000394601.8 3185 550aa Protein coding CCDS45892 P26599-2 - TSL:1, GENCODE basic,
PTBP1-201 ENST00000349038.8 3155 531aa Protein coding CCDS32859 P26599-1 - TSL:1, GENCODE basic, APPRIS ALT1,
PTBP1-220 ENST00000635647.1 3691 588aa Protein coding - A0A0U1RRM4 - TSL:5, GENCODE basic,
PTBP1-224 ENST00000679114.1 3233 559aa Protein coding - - - GENCODE basic,
PTBP1-202 ENST00000350092.8 3069 527aa Protein coding - A6NLN1 - TSL:1, GENCODE basic,
PTBP1-219 ENST00000627714.2 2201 197aa Protein coding - A0A0D9SF20 - TSL:5, GENCODE basic,
PTBP1-208 ENST00000585956.5 767 256aa Protein coding - K7EK45 - CDS 5' and 3' incomplete, TSL:3,
PTBP1-213 ENST00000589575.5 749 237aa Protein coding - K7EKJ7 - CDS 3' incomplete, TSL:3,
PTBP1-209 ENST00000586481.5 578 149aa Protein coding - K7ES59 - CDS 3' incomplete, TSL:3,
PTBP1-212 ENST00000587191.3 357 81aa Protein coding - A0A087WUW5 - CDS 3' incomplete, TSL:5,
PTBP1-205 ENST00000585535.2 314 105aa Protein coding - K7ELW5 - CDS 5' and 3' incomplete, TSL:3,
PTBP1-221 ENST00000676227.1 3275 339aa Nonsense mediated decay - - - -
PTBP1-222 ENST00000677277.1 3194 313aa Nonsense mediated decay - - - -
PTBP1-218 ENST00000621737.4 1125 227aa Nonsense mediated decay - A0A087WU68 - CDS 5' incomplete, TSL:5,
PTBP1-207 ENST00000585932.5 1030 115aa Nonsense mediated decay - A0A087WTS6 - CDS 5' incomplete, TSL:3,
PTBP1-210 ENST00000586944.5 4661 No protein Retained intron - - - TSL:2,
PTBP1-217 ENST00000592804.2 1556 No protein Retained intron - - - TSL:2,
PTBP1-216 ENST00000592113.5 1548 No protein Retained intron - - - TSL:5,
PTBP1-206 ENST00000585856.2 1217 No protein Retained intron - - - TSL:2,
PTBP1-223 ENST00000677717.1 723 No protein Retained intron - - - -
PTBP1-214 ENST00000589883.1 683 No protein Retained intron - - - TSL:3,
PTBP1-215 ENST00000590887.5 642 No protein Retained intron - - - TSL:3,
PTBP1-211 ENST00000587136.1 583 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PTBP1
This KO Strategy loading
Red Cotton™ Notes Gene PTBP1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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