CRISPR-U™ Gene Knockout Cell Line
Strategy
PSME1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PSME1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PSME1 |
| Gene id | 5720 |
| Organism | Homo sapiens |
| Official full symbol | proteasome activator subunit 1 |
| Gene type | protein-coding |
| Also known as | HEL-S-129m, IFI5111, PA28A, PA28alpha, REGalpha |
| Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PSME1-202 | ENST00000382708.7 | 1136 | 250aa | Protein coding | CCDS41930 | Q06323-2 | - | TSL:2, GENCODE basic, |
| PSME1-201 | ENST00000206451.11 | 965 | 249aa | Protein coding | CCDS9612 | Q06323-1 | NM_006263.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PSME1-210 | ENST00000561435.5 | 945 | 233aa | Protein coding | CCDS61415 | Q06323-3 | - | TSL:2, GENCODE basic, |
| PSME1-205 | ENST00000559123.5 | 1033 | 90aa | Protein coding | - | H0YKK6 | - | TSL:5, GENCODE basic, |
| PSME1-204 | ENST00000558112.1 | 651 | 114aa | Nonsense mediated decay | - | H0YLU2 | - | CDS 5' incomplete, TSL:5, |
| PSME1-203 | ENST00000470718.5 | 476 | No protein | Processed transcript | - | - | - | TSL:3, |
| PSME1-208 | ENST00000561059.5 | 947 | No protein | Retained intron | - | - | - | TSL:2, |
| PSME1-207 | ENST00000560420.1 | 922 | No protein | Retained intron | - | - | - | TSL:2, |
| PSME1-209 | ENST00000561142.5 | 865 | No protein | Retained intron | - | - | - | TSL:5, |
| PSME1-206 | ENST00000559741.5 | 763 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PSME1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PSME1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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