CRISPR-U™ Gene Knockout Cell Line Strategy

PSME1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PSME1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PSME1
Gene id 5720
Organism Homo sapiens
Official full symbol proteasome activator subunit 1
Gene type protein-coding
Also known as HEL-S-129m, IFI5111, PA28A, PA28alpha, REGalpha
Summary The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 14
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PSME1-202 ENST00000382708.7 1136 250aa Protein coding CCDS41930 Q06323-2 - TSL:2, GENCODE basic,
PSME1-201 ENST00000206451.11 965 249aa Protein coding CCDS9612 Q06323-1 NM_006263.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PSME1-210 ENST00000561435.5 945 233aa Protein coding CCDS61415 Q06323-3 - TSL:2, GENCODE basic,
PSME1-205 ENST00000559123.5 1033 90aa Protein coding - H0YKK6 - TSL:5, GENCODE basic,
PSME1-204 ENST00000558112.1 651 114aa Nonsense mediated decay - H0YLU2 - CDS 5' incomplete, TSL:5,
PSME1-203 ENST00000470718.5 476 No protein Processed transcript - - - TSL:3,
PSME1-208 ENST00000561059.5 947 No protein Retained intron - - - TSL:2,
PSME1-207 ENST00000560420.1 922 No protein Retained intron - - - TSL:2,
PSME1-209 ENST00000561142.5 865 No protein Retained intron - - - TSL:5,
PSME1-206 ENST00000559741.5 763 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PSME1
This KO Strategy loading
Red Cotton™ Notes Gene PSME1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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