CRISPR-U™ Gene Knockout Cell Line Strategy

BAD Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BAD Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BAD
Gene id 572
Organism Homo sapiens
Official full symbol BCL2 associated agonist of cell death
Gene type protein-coding
Also known as BBC2, BCL2L8
Summary The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Genomic regions Chromosome 11
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BAD-203 ENST00000394532.7 1157 168aa Protein coding CCDS8065 A0A024R562 Q92934 - TSL:1, GENCODE basic, APPRIS P1,
BAD-201 ENST00000309032.8 929 168aa Protein coding CCDS8065 A0A024R562 Q92934 NM_032989.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
BAD-202 ENST00000394531.3 631 163aa Protein coding - A8MXU7 - TSL:3, GENCODE basic,
BAD-207 ENST00000544785.1 525 157aa Protein coding - F5GYS3 - TSL:3, GENCODE basic,
BAD-204 ENST00000492141.1 359 51aa Protein coding - F5H3B1 - CDS 3' incomplete, TSL:5,
BAD-205 ENST00000493798.1 325 64aa Protein coding - F5H1R6 - CDS 3' incomplete, TSL:2,
BAD-206 ENST00000544271.1 552 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BAD
This KO Strategy loading
Red Cotton™ Notes Gene BAD had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×