CRISPR-U™ Gene Knockout Cell Line
Strategy
BAD Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BAD Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BAD |
| Gene id | 572 |
| Organism | Homo sapiens |
| Official full symbol | BCL2 associated agonist of cell death |
| Gene type | protein-coding |
| Also known as | BBC2, BCL2L8 |
| Summary | The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BAD-203 | ENST00000394532.7 | 1157 | 168aa | Protein coding | CCDS8065 | A0A024R562 Q92934 | - | TSL:1, GENCODE basic, APPRIS P1, |
| BAD-201 | ENST00000309032.8 | 929 | 168aa | Protein coding | CCDS8065 | A0A024R562 Q92934 | NM_032989.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| BAD-202 | ENST00000394531.3 | 631 | 163aa | Protein coding | - | A8MXU7 | - | TSL:3, GENCODE basic, |
| BAD-207 | ENST00000544785.1 | 525 | 157aa | Protein coding | - | F5GYS3 | - | TSL:3, GENCODE basic, |
| BAD-204 | ENST00000492141.1 | 359 | 51aa | Protein coding | - | F5H3B1 | - | CDS 3' incomplete, TSL:5, |
| BAD-205 | ENST00000493798.1 | 325 | 64aa | Protein coding | - | F5H1R6 | - | CDS 3' incomplete, TSL:2, |
| BAD-206 | ENST00000544271.1 | 552 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BAD |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BAD had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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