CRISPR-U™ Gene Knockout Cell Line Strategy

PSMD10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PSMD10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PSMD10
Gene id 5716
Organism Homo sapiens
Official full symbol proteasome 26S subunit, non-ATPase 10
Gene type protein-coding
Also known as dJ889N15.2, p28, p28(GANK)
Summary This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.
Genomic regions Chromosome X
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PSMD10-201 ENST00000217958.8 1470 226aa Protein coding CCDS14536 O75832-1 NM_002814.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PSMD10-204 ENST00000361815.9 768 151aa Protein coding CCDS14537 O75832-2 - TSL:1, GENCODE basic,
PSMD10-206 ENST00000372296.5 878 110aa Protein coding - B1AJY6 - TSL:5, GENCODE basic,
PSMD10-205 ENST00000372295.5 779 185aa Protein coding - B1AJY5 - TSL:5, GENCODE basic,
PSMD10-203 ENST00000340200.5 713 193aa Protein coding - B1AJY7 - TSL:3, GENCODE basic,
PSMD10-207 ENST00000553388.1 1039 No protein Retained intron - - - TSL:1,
PSMD10-202 ENST00000338548.4 514 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PSMD10
This KO Strategy loading
Red Cotton™ Notes Gene PSMD10 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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