CRISPR-U™ Gene Knockout Cell Line
Strategy
PSMD10 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PSMD10 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PSMD10 |
| Gene id | 5716 |
| Organism | Homo sapiens |
| Official full symbol | proteasome 26S subunit, non-ATPase 10 |
| Gene type | protein-coding |
| Also known as | dJ889N15.2, p28, p28(GANK) |
| Summary | This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PSMD10-201 | ENST00000217958.8 | 1470 | 226aa | Protein coding | CCDS14536 | O75832-1 | NM_002814.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PSMD10-204 | ENST00000361815.9 | 768 | 151aa | Protein coding | CCDS14537 | O75832-2 | - | TSL:1, GENCODE basic, |
| PSMD10-206 | ENST00000372296.5 | 878 | 110aa | Protein coding | - | B1AJY6 | - | TSL:5, GENCODE basic, |
| PSMD10-205 | ENST00000372295.5 | 779 | 185aa | Protein coding | - | B1AJY5 | - | TSL:5, GENCODE basic, |
| PSMD10-203 | ENST00000340200.5 | 713 | 193aa | Protein coding | - | B1AJY7 | - | TSL:3, GENCODE basic, |
| PSMD10-207 | ENST00000553388.1 | 1039 | No protein | Retained intron | - | - | - | TSL:1, |
| PSMD10-202 | ENST00000338548.4 | 514 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PSMD10 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PSMD10 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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