CRISPR-U™ Gene Knockout Cell Line
Strategy
PSMB10 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PSMB10 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PSMB10 |
| Gene id | 5699 |
| Organism | Homo sapiens |
| Official full symbol | proteasome 20S subunit beta 10 |
| Gene type | protein-coding |
| Also known as | LMP10, MECL1, beta2i |
| Summary | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PSMB10-201 | ENST00000358514.9 | 977 | 273aa | Protein coding | CCDS10853 | P40306 | NM_002801.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PSMB10-204 | ENST00000574576.2 | 539 | 138aa | Protein coding | - | J3QQN1 | - | CDS 5' incomplete, TSL:3, |
| PSMB10-205 | ENST00000575556.1 | 966 | No protein | Retained intron | - | - | - | TSL:2, |
| PSMB10-202 | ENST00000570304.1 | 851 | No protein | Retained intron | - | - | - | TSL:2, |
| PSMB10-203 | ENST00000570985.1 | 591 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PSMB10 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PSMB10 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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