CRISPR-U™ Gene Knockout Cell Line Strategy

PSMB10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PSMB10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PSMB10
Gene id 5699
Organism Homo sapiens
Official full symbol proteasome 20S subunit beta 10
Gene type protein-coding
Also known as LMP10, MECL1, beta2i
Summary The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
Genomic regions Chromosome 16
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PSMB10-201 ENST00000358514.9 977 273aa Protein coding CCDS10853 P40306 NM_002801.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PSMB10-204 ENST00000574576.2 539 138aa Protein coding - J3QQN1 - CDS 5' incomplete, TSL:3,
PSMB10-205 ENST00000575556.1 966 No protein Retained intron - - - TSL:2,
PSMB10-202 ENST00000570304.1 851 No protein Retained intron - - - TSL:2,
PSMB10-203 ENST00000570985.1 591 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PSMB10
This KO Strategy loading
Red Cotton™ Notes Gene PSMB10 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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