CRISPR-U™ Gene Knockout Cell Line
Strategy
PSMB8 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PSMB8 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PSMB8 |
| Gene id | 5696 |
| Organism | Homo sapiens |
| Official full symbol | proteasome 20S subunit beta 8 |
| Gene type | protein-coding |
| Also known as | ALDD, D6S216, D6S216E, JMP, LMP7, NKJO, PRAAS1, PSMB5i, RING10 |
| Summary | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PSMB8-201 | ENST00000374881.3 | 1327 | 272aa | Protein coding | CCDS4756 | P28062-2 | - | TSL:1, GENCODE basic, |
| PSMB8-202 | ENST00000374882.8 | 1124 | 276aa | Protein coding | CCDS4757 | P28062-1 | NM_148919.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PSMB8-203 | ENST00000395339.7 | 1025 | 252aa | Protein coding | - | Q5JNW7 | - | TSL:3, GENCODE basic, |
| PSMB8-206 | ENST00000650411.1 | 2373 | No protein | Retained intron | - | - | - | - |
| PSMB8-207 | ENST00000650793.1 | 1335 | No protein | Retained intron | - | - | - | - |
| PSMB8-204 | ENST00000484003.1 | 1153 | No protein | Retained intron | - | - | - | TSL:5, |
| PSMB8-205 | ENST00000490613.1 | 835 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PSMB8 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PSMB8 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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