CRISPR-U™ Gene Knockout Cell Line Strategy

PSMB8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PSMB8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PSMB8
Gene id 5696
Organism Homo sapiens
Official full symbol proteasome 20S subunit beta 8
Gene type protein-coding
Also known as ALDD, D6S216, D6S216E, JMP, LMP7, NKJO, PRAAS1, PSMB5i, RING10
Summary The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
Genomic regions Chromosome 6
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PSMB8-201 ENST00000374881.3 1327 272aa Protein coding CCDS4756 P28062-2 - TSL:1, GENCODE basic,
PSMB8-202 ENST00000374882.8 1124 276aa Protein coding CCDS4757 P28062-1 NM_148919.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PSMB8-203 ENST00000395339.7 1025 252aa Protein coding - Q5JNW7 - TSL:3, GENCODE basic,
PSMB8-206 ENST00000650411.1 2373 No protein Retained intron - - - -
PSMB8-207 ENST00000650793.1 1335 No protein Retained intron - - - -
PSMB8-204 ENST00000484003.1 1153 No protein Retained intron - - - TSL:5,
PSMB8-205 ENST00000490613.1 835 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PSMB8
This KO Strategy loading
Red Cotton™ Notes Gene PSMB8 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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