CRISPR-U™ Gene Knockout Cell Line Strategy

OTUD7B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human OTUD7B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol OTUD7B
Gene id 56957
Organism Homo sapiens
Official full symbol OTU deubiquitinase 7B
Gene type protein-coding
Also known as CEZANNE, ZA20D1
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
OTUD7B-202 ENST00000581312.6 8922 843aa Protein coding CCDS72903 Q6GQQ9-1 NM_020205.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
OTUD7B-201 ENST00000417191.2 1425 427aa Protein coding - Q5SZ59 - CDS 3' incomplete, TSL:1,
OTUD7B-203 ENST00000619871.1 3818 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene OTUD7B
This KO Strategy loading
Red Cotton™ Notes Gene OTUD7B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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