CRISPR-U™ Gene Knockout Cell Line
Strategy
CMC2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CMC2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CMC2 |
| Gene id | 56942 |
| Organism | Homo sapiens |
| Official full symbol | C-X9-C motif containing 2 |
| Gene type | protein-coding |
| Also known as | 2310061C15Rik, C16orf61, DC13 |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CMC2-201 | ENST00000219400.8 | 10072 | 79aa | Protein coding | CCDS10930 | Q9NRP2 | NM_020188.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CMC2-208 | ENST00000564249.5 | 890 | 79aa | Protein coding | CCDS10930 | Q9NRP2 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CMC2-212 | ENST00000565650.5 | 671 | 79aa | Protein coding | CCDS10930 | Q9NRP2 | - | TSL:2, GENCODE basic, APPRIS P1, |
| CMC2-213 | ENST00000565914.5 | 528 | 79aa | Protein coding | CCDS10930 | Q9NRP2 | - | TSL:3, GENCODE basic, APPRIS P1, |
| CMC2-223 | ENST00000570195.5 | 871 | 93aa | Protein coding | - | H3BTB6 | - | TSL:3, GENCODE basic, |
| CMC2-202 | ENST00000486645.5 | 766 | 50aa | Protein coding | - | H3BRC3 | - | TSL:3, GENCODE basic, |
| CMC2-214 | ENST00000565925.5 | 640 | 74aa | Protein coding | - | H3BNN8 | - | TSL:5, GENCODE basic, |
| CMC2-204 | ENST00000562713.3 | 573 | 74aa | Protein coding | - | H3BNN8 | - | TSL:3, GENCODE basic, |
| CMC2-224 | ENST00000630396.1 | 481 | 93aa | Protein coding | - | H3BTB6 | - | TSL:5, GENCODE basic, |
| CMC2-207 | ENST00000564174.5 | 424 | 55aa | Protein coding | - | H3BPP2 | - | TSL:3, GENCODE basic, |
| CMC2-209 | ENST00000565108.5 | 399 | 55aa | Protein coding | - | H3BPP2 | - | TSL:2, GENCODE basic, |
| CMC2-218 | ENST00000567593.5 | 919 | 36aa | Nonsense mediated decay | - | H3BN28 | - | TSL:3, |
| CMC2-219 | ENST00000568228.5 | 825 | 51aa | Nonsense mediated decay | - | H3BP68 | - | TSL:3, |
| CMC2-222 | ENST00000570086.5 | 801 | 51aa | Nonsense mediated decay | - | H3BP68 | - | TSL:3, |
| CMC2-203 | ENST00000561614.5 | 683 | 54aa | Nonsense mediated decay | - | H3BQQ1 | - | CDS 5' incomplete, TSL:3, |
| CMC2-211 | ENST00000565613.5 | 663 | 74aa | Nonsense mediated decay | - | H3BNN8 | - | TSL:5, |
| CMC2-216 | ENST00000566231.5 | 652 | No protein | Processed transcript | - | - | - | TSL:3, |
| CMC2-220 | ENST00000569187.5 | 612 | No protein | Processed transcript | - | - | - | TSL:2, |
| CMC2-217 | ENST00000566918.5 | 605 | No protein | Processed transcript | - | - | - | TSL:3, |
| CMC2-205 | ENST00000562828.5 | 438 | No protein | Processed transcript | - | - | - | TSL:2, |
| CMC2-210 | ENST00000565514.1 | 262 | No protein | Processed transcript | - | - | - | TSL:3, |
| CMC2-206 | ENST00000563779.5 | 2087 | No protein | Retained intron | - | - | - | TSL:1, |
| CMC2-215 | ENST00000566047.5 | 660 | No protein | Retained intron | - | - | - | TSL:2, |
| CMC2-221 | ENST00000569666.5 | 545 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CMC2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CMC2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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