CRISPR-U™ Gene Knockout Cell Line Strategy

PSAP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PSAP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PSAP
Gene id 5660
Organism Homo sapiens
Official full symbol prosaposin
Gene type protein-coding
Also known as GLBA, SAP1, SAP2
Summary This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Genomic regions Chromosome 10
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PSAP-202 ENST00000394936.8 2748 524aa Protein coding CCDS7311 P07602-1 NM_002778.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
PSAP-201 ENST00000394934.4 2830 527aa Protein coding - C9JIZ6 - TSL:5, GENCODE basic, APPRIS ALT2,
PSAP-205 ENST00000610929.3 1969 240aa Protein coding - Q5BJH1 - TSL:1, GENCODE basic,
PSAP-206 ENST00000633965.1 500 167aa Protein coding - A0A0J9YXB8 - CDS 5' and 3' incomplete, TSL:5,
PSAP-203 ENST00000493143.1 576 No protein Retained intron - - - TSL:2,
PSAP-204 ENST00000495196.1 344 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PSAP
This KO Strategy loading
Red Cotton™ Notes Gene PSAP had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×