CRISPR-U™ Gene Knockout Cell Line
Strategy
MAPK6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAPK6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAPK6 |
| Gene id | 5597 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase 6 |
| Gene type | protein-coding |
| Also known as | ERK3, HsT17250, PRKM6, p97MAPK |
| Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAPK6-212 | ENST00000681888.1 | 5338 | 721aa | Protein coding | CCDS10147 | - | - | GENCODE basic, APPRIS P1, |
| MAPK6-201 | ENST00000261845.7 | 5330 | 721aa | Protein coding | CCDS10147 | Q16659 | NM_002748.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MAPK6-209 | ENST00000680066.1 | 5299 | 721aa | Protein coding | CCDS10147 | - | - | GENCODE basic, APPRIS P1, |
| MAPK6-210 | ENST00000680652.1 | 5288 | 721aa | Protein coding | CCDS10147 | - | - | GENCODE basic, APPRIS P1, |
| MAPK6-211 | ENST00000680777.1 | 5287 | 721aa | Protein coding | CCDS10147 | - | - | GENCODE basic, APPRIS P1, |
| MAPK6-204 | ENST00000558841.1 | 656 | No protein | Processed transcript | - | - | - | TSL:2, |
| MAPK6-202 | ENST00000558063.5 | 583 | No protein | Processed transcript | - | - | - | TSL:4, |
| MAPK6-205 | ENST00000558891.1 | 583 | No protein | Processed transcript | - | - | - | TSL:3, |
| MAPK6-206 | ENST00000560254.1 | 574 | No protein | Processed transcript | - | - | - | TSL:4, |
| MAPK6-207 | ENST00000560774.1 | 574 | No protein | Processed transcript | - | - | - | TSL:4, |
| MAPK6-208 | ENST00000560802.1 | 552 | No protein | Processed transcript | - | - | - | TSL:4, |
| MAPK6-203 | ENST00000558100.1 | 514 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAPK6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAPK6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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