CRISPR-U™ Gene Knockout Cell Line Strategy

PRKCSH Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PRKCSH Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRKCSH
Gene id 5589
Organism Homo sapiens
Official full symbol protein kinase C substrate 80K-H
Gene type protein-coding
Also known as AGE-R2, G19P1, GIIB, PCLD, PCLD1, PKCSH, PLD1, VASAP-60
Summary This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 19
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRKCSH-205 ENST00000587327.5 2189 525aa Protein coding CCDS45977 P14314-2 - TSL:2, GENCODE basic, APPRIS ALT2,
PRKCSH-217 ENST00000592741.5 2100 535aa Protein coding CCDS74286 K7ELL7 - TSL:1, GENCODE basic, APPRIS ALT2,
PRKCSH-222 ENST00000677123.1 2096 535aa Protein coding CCDS74286 - NM_001289104.2 GENCODE basic, APPRIS ALT2, MANE Select v0.92,
PRKCSH-212 ENST00000591462.6 2063 525aa Protein coding CCDS45977 P14314-2 - TSL:5, GENCODE basic, APPRIS ALT2,
PRKCSH-209 ENST00000589838.5 1898 528aa Protein coding CCDS32911 P14314-1 - TSL:1, GENCODE basic, APPRIS P4,
PRKCSH-221 ENST00000676823.1 814 171aa Protein coding - - - CDS 3' incomplete,
PRKCSH-208 ENST00000589126.5 625 168aa Protein coding - K7EPW7 - CDS 3' incomplete, TSL:5,
PRKCSH-219 ENST00000593101.5 578 144aa Protein coding - K7EJ70 - CDS 3' incomplete, TSL:4,
PRKCSH-214 ENST00000591946.5 569 62aa Protein coding - K7EIP3 - CDS 3' incomplete, TSL:2,
PRKCSH-207 ENST00000588269.1 557 132aa Protein coding - K7EL27 - CDS 3' incomplete, TSL:5,
PRKCSH-206 ENST00000587509.5 545 140aa Protein coding - K7EKX1 - CDS 3' incomplete, TSL:4,
PRKCSH-203 ENST00000586486.1 1613 200aa Nonsense mediated decay - A0A0C4DGP4 - CDS 5' incomplete, TSL:1,
PRKCSH-213 ENST00000591510.1 591 No protein Processed transcript - - - TSL:3,
PRKCSH-218 ENST00000593053.1 588 No protein Processed transcript - - - TSL:5,
PRKCSH-220 ENST00000593104.5 545 No protein Processed transcript - - - TSL:4,
PRKCSH-211 ENST00000590098.1 795 No protein Retained intron - - - TSL:2,
PRKCSH-204 ENST00000587290.5 780 No protein Retained intron - - - TSL:2,
PRKCSH-201 ENST00000585325.5 598 No protein Retained intron - - - TSL:4,
PRKCSH-216 ENST00000592445.1 598 No protein Retained intron - - - TSL:2,
PRKCSH-210 ENST00000589990.1 567 No protein Retained intron - - - TSL:2,
PRKCSH-202 ENST00000585540.1 501 No protein Retained intron - - - TSL:3,
PRKCSH-215 ENST00000592435.1 358 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRKCSH
This KO Strategy loading
Red Cotton™ Notes Gene PRKCSH had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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