CRISPR-U™ Gene Knockout Cell Line
Strategy
PRKCSH Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRKCSH Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PRKCSH |
| Gene id | 5589 |
| Organism | Homo sapiens |
| Official full symbol | protein kinase C substrate 80K-H |
| Gene type | protein-coding |
| Also known as | AGE-R2, G19P1, GIIB, PCLD, PCLD1, PKCSH, PLD1, VASAP-60 |
| Summary | This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PRKCSH-205 | ENST00000587327.5 | 2189 | 525aa | Protein coding | CCDS45977 | P14314-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| PRKCSH-217 | ENST00000592741.5 | 2100 | 535aa | Protein coding | CCDS74286 | K7ELL7 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PRKCSH-222 | ENST00000677123.1 | 2096 | 535aa | Protein coding | CCDS74286 | - | NM_001289104.2 | GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| PRKCSH-212 | ENST00000591462.6 | 2063 | 525aa | Protein coding | CCDS45977 | P14314-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| PRKCSH-209 | ENST00000589838.5 | 1898 | 528aa | Protein coding | CCDS32911 | P14314-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PRKCSH-221 | ENST00000676823.1 | 814 | 171aa | Protein coding | - | - | - | CDS 3' incomplete, |
| PRKCSH-208 | ENST00000589126.5 | 625 | 168aa | Protein coding | - | K7EPW7 | - | CDS 3' incomplete, TSL:5, |
| PRKCSH-219 | ENST00000593101.5 | 578 | 144aa | Protein coding | - | K7EJ70 | - | CDS 3' incomplete, TSL:4, |
| PRKCSH-214 | ENST00000591946.5 | 569 | 62aa | Protein coding | - | K7EIP3 | - | CDS 3' incomplete, TSL:2, |
| PRKCSH-207 | ENST00000588269.1 | 557 | 132aa | Protein coding | - | K7EL27 | - | CDS 3' incomplete, TSL:5, |
| PRKCSH-206 | ENST00000587509.5 | 545 | 140aa | Protein coding | - | K7EKX1 | - | CDS 3' incomplete, TSL:4, |
| PRKCSH-203 | ENST00000586486.1 | 1613 | 200aa | Nonsense mediated decay | - | A0A0C4DGP4 | - | CDS 5' incomplete, TSL:1, |
| PRKCSH-213 | ENST00000591510.1 | 591 | No protein | Processed transcript | - | - | - | TSL:3, |
| PRKCSH-218 | ENST00000593053.1 | 588 | No protein | Processed transcript | - | - | - | TSL:5, |
| PRKCSH-220 | ENST00000593104.5 | 545 | No protein | Processed transcript | - | - | - | TSL:4, |
| PRKCSH-211 | ENST00000590098.1 | 795 | No protein | Retained intron | - | - | - | TSL:2, |
| PRKCSH-204 | ENST00000587290.5 | 780 | No protein | Retained intron | - | - | - | TSL:2, |
| PRKCSH-201 | ENST00000585325.5 | 598 | No protein | Retained intron | - | - | - | TSL:4, |
| PRKCSH-216 | ENST00000592445.1 | 598 | No protein | Retained intron | - | - | - | TSL:2, |
| PRKCSH-210 | ENST00000589990.1 | 567 | No protein | Retained intron | - | - | - | TSL:2, |
| PRKCSH-202 | ENST00000585540.1 | 501 | No protein | Retained intron | - | - | - | TSL:3, |
| PRKCSH-215 | ENST00000592435.1 | 358 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PRKCSH |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PRKCSH had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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