CRISPR-U™ Gene Knockout Cell Line
Strategy
ASH1L Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ASH1L Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ASH1L |
| Gene id | 55870 |
| Organism | Homo sapiens |
| Official full symbol | ASH1 like histone lysine methyltransferase |
| Gene type | protein-coding |
| Also known as | ASH1, ASH1L1, KMT2H, MRD52 |
| Summary | This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ASH1L-202 | ENST00000392403.8 | 12037 | 2964aa | Protein coding | CCDS1113 | Q9NR48-2 | NM_018489.3 | TSL:5, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ASH1L-213 | ENST00000678117.1 | 11549 | 2964aa | Protein coding | CCDS1113 | - | - | GENCODE basic, APPRIS P2, |
| ASH1L-215 | ENST00000679097.1 | 11530 | 2964aa | Protein coding | CCDS1113 | - | - | GENCODE basic, APPRIS P2, |
| ASH1L-210 | ENST00000677213.1 | 11457 | 2964aa | Protein coding | CCDS1113 | - | - | GENCODE basic, APPRIS P2, |
| ASH1L-201 | ENST00000368346.7 | 11942 | 2969aa | Protein coding | - | Q9NR48-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| ASH1L-216 | ENST00000679133.1 | 11431 | 2969aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ASH1L-211 | ENST00000677825.1 | 11364 | 2963aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ASH1L-217 | ENST00000679333.1 | 11250 | 2904aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| ASH1L-212 | ENST00000677992.1 | 3377 | 339aa | Protein coding | - | - | - | CDS 5' incomplete, |
| ASH1L-208 | ENST00000676814.1 | 12184 | 1709aa | Nonsense mediated decay | - | - | - | - |
| ASH1L-209 | ENST00000677042.1 | 11471 | 1726aa | Nonsense mediated decay | - | - | - | - |
| ASH1L-214 | ENST00000678699.1 | 11458 | 1741aa | Nonsense mediated decay | - | - | - | - |
| ASH1L-207 | ENST00000548830.2 | 5489 | 177aa | Nonsense mediated decay | - | F8VWK7 | - | TSL:5, |
| ASH1L-205 | ENST00000492987.2 | 784 | 154aa | Nonsense mediated decay | - | H0YI82 | - | CDS 5' incomplete, TSL:5, |
| ASH1L-204 | ENST00000478837.5 | 723 | No protein | Retained intron | - | - | - | TSL:2, |
| ASH1L-203 | ENST00000477427.1 | 596 | No protein | Retained intron | - | - | - | TSL:2, |
| ASH1L-206 | ENST00000548566.1 | 584 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ASH1L |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ASH1L had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: