CRISPR-U™ Gene Knockout Cell Line Strategy

ASH1L Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ASH1L Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ASH1L
Gene id 55870
Organism Homo sapiens
Official full symbol ASH1 like histone lysine methyltransferase
Gene type protein-coding
Also known as ASH1, ASH1L1, KMT2H, MRD52
Summary This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions.
Genomic regions Chromosome 1
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ASH1L-202 ENST00000392403.8 12037 2964aa Protein coding CCDS1113 Q9NR48-2 NM_018489.3 TSL:5, GENCODE basic, APPRIS P2, MANE Select v0.92,
ASH1L-213 ENST00000678117.1 11549 2964aa Protein coding CCDS1113 - - GENCODE basic, APPRIS P2,
ASH1L-215 ENST00000679097.1 11530 2964aa Protein coding CCDS1113 - - GENCODE basic, APPRIS P2,
ASH1L-210 ENST00000677213.1 11457 2964aa Protein coding CCDS1113 - - GENCODE basic, APPRIS P2,
ASH1L-201 ENST00000368346.7 11942 2969aa Protein coding - Q9NR48-1 - TSL:1, GENCODE basic, APPRIS ALT2,
ASH1L-216 ENST00000679133.1 11431 2969aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ASH1L-211 ENST00000677825.1 11364 2963aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ASH1L-217 ENST00000679333.1 11250 2904aa Protein coding - - - GENCODE basic, APPRIS ALT2,
ASH1L-212 ENST00000677992.1 3377 339aa Protein coding - - - CDS 5' incomplete,
ASH1L-208 ENST00000676814.1 12184 1709aa Nonsense mediated decay - - - -
ASH1L-209 ENST00000677042.1 11471 1726aa Nonsense mediated decay - - - -
ASH1L-214 ENST00000678699.1 11458 1741aa Nonsense mediated decay - - - -
ASH1L-207 ENST00000548830.2 5489 177aa Nonsense mediated decay - F8VWK7 - TSL:5,
ASH1L-205 ENST00000492987.2 784 154aa Nonsense mediated decay - H0YI82 - CDS 5' incomplete, TSL:5,
ASH1L-204 ENST00000478837.5 723 No protein Retained intron - - - TSL:2,
ASH1L-203 ENST00000477427.1 596 No protein Retained intron - - - TSL:2,
ASH1L-206 ENST00000548566.1 584 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ASH1L
This KO Strategy loading
Red Cotton™ Notes Gene ASH1L had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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