CRISPR-U™ Gene Knockout Cell Line
Strategy
HDAC8 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HDAC8 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HDAC8 |
| Gene id | 55869 |
| Organism | Homo sapiens |
| Official full symbol | histone deacetylase 8 |
| Gene type | protein-coding |
| Also known as | CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS |
| Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 30 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HDAC8-214 | ENST00000439122.7 | 2373 | 256aa | Protein coding | CCDS55451 | Q9BY41-5 | - | TSL:2, GENCODE basic, |
| HDAC8-207 | ENST00000373573.9 | 1754 | 377aa | Protein coding | CCDS14420 | Q9BY41-1 | NM_018486.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| HDAC8-209 | ENST00000373589.9 | 1695 | 286aa | Protein coding | CCDS55449 | Q9BY41-4 | - | TSL:2, GENCODE basic, |
| HDAC8-201 | ENST00000373554.6 | 836 | 146aa | Protein coding | CCDS55450 | Q9BY41-8 | - | TSL:2, GENCODE basic, |
| HDAC8-202 | ENST00000373556.8 | 747 | 158aa | Protein coding | CCDS55452 | Q9BY41-7 | - | TSL:2, GENCODE basic, |
| HDAC8-203 | ENST00000373559.8 | 632 | 139aa | Protein coding | CCDS55448 | Q9BY41-6 | - | TSL:2, GENCODE basic, |
| HDAC8-234 | ENST00000648298.1 | 5689 | 350aa | Protein coding | - | A0A3B3IRP8 | - | GENCODE basic, |
| HDAC8-254 | ENST00000649752.1 | 5197 | 259aa | Protein coding | - | A0A3B3ISE4 | - | GENCODE basic, |
| HDAC8-244 | ENST00000648870.1 | 4155 | 337aa | Protein coding | - | A0A3B3IT84 | - | GENCODE basic, |
| HDAC8-224 | ENST00000647859.1 | 2502 | 373aa | Protein coding | - | A0A3B3IRI9 | - | GENCODE basic, APPRIS ALT2, |
| HDAC8-206 | ENST00000373571.6 | 2135 | 253aa | Protein coding | - | A6NMT1 | - | TSL:5, GENCODE basic, |
| HDAC8-228 | ENST00000648036.1 | 2079 | 254aa | Protein coding | - | A0A3B3IRZ8 | - | GENCODE basic, |
| HDAC8-256 | ENST00000650126.1 | 2044 | 230aa | Protein coding | - | A0A3B3IU01 | - | GENCODE basic, |
| HDAC8-246 | ENST00000648962.1 | 1924 | 206aa | Protein coding | - | A0A3B3ISY0 | - | GENCODE basic, |
| HDAC8-235 | ENST00000648452.1 | 1865 | 414aa | Protein coding | - | A0A3B3ISJ4 | - | GENCODE basic, APPRIS ALT2, |
| HDAC8-225 | ENST00000647886.1 | 1807 | 403aa | Protein coding | - | A0A3B3IS68 | - | GENCODE basic, APPRIS ALT2, |
| HDAC8-219 | ENST00000647594.1 | 1785 | 373aa | Protein coding | - | A0A3B3IRI9 | - | GENCODE basic, APPRIS ALT2, |
| HDAC8-208 | ENST00000373583.6 | 1710 | 351aa | Protein coding | - | A6NFW1 | - | TSL:5, GENCODE basic, |
| HDAC8-227 | ENST00000647980.1 | 1700 | 375aa | Protein coding | - | A0A3B3ITZ3 | - | GENCODE basic, APPRIS ALT2, |
| HDAC8-205 | ENST00000373568.7 | 1625 | 417aa | Protein coding | - | A6NGJ7 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| HDAC8-222 | ENST00000647654.1 | 1516 | 312aa | Protein coding | - | A0A3B3IT30 | - | GENCODE basic, |
| HDAC8-259 | ENST00000650604.1 | 1367 | 186aa | Protein coding | - | A0A3B3IS37 | - | GENCODE basic, |
| HDAC8-211 | ENST00000415409.6 | 1274 | 344aa | Protein coding | - | E7EVA8 | - | TSL:5, GENCODE basic, |
| HDAC8-230 | ENST00000648139.1 | 1220 | 317aa | Protein coding | - | A0A3B3IRJ3 | - | GENCODE basic, |
| HDAC8-236 | ENST00000648459.1 | 1026 | 176aa | Protein coding | - | A0A3B3IU52 | - | CDS 5' incomplete, |
| HDAC8-255 | ENST00000650076.1 | 971 | 163aa | Protein coding | - | A0A3B3IRZ0 | - | CDS 5' incomplete, |
| HDAC8-204 | ENST00000373560.7 | 829 | 156aa | Protein coding | - | A6NGT0 | - | TSL:3, GENCODE basic, |
| HDAC8-260 | ENST00000650636.1 | 747 | 150aa | Protein coding | - | A0A3B3IS93 | - | GENCODE basic, |
| HDAC8-232 | ENST00000648276.1 | 652 | 167aa | Protein coding | - | A0A3B3IT98 | - | CDS 5' incomplete, |
| HDAC8-212 | ENST00000421523.6 | 552 | 129aa | Protein coding | - | C9J8F0 | - | CDS 3' incomplete, TSL:3, |
| HDAC8-248 | ENST00000649116.1 | 5673 | 146aa | Nonsense mediated decay | - | Q9BY41-8 | - | - |
| HDAC8-238 | ENST00000648577.1 | 5060 | 193aa | Nonsense mediated decay | - | A0A3B3IRJ8 | - | - |
| HDAC8-250 | ENST00000649242.1 | 3072 | 193aa | Nonsense mediated decay | - | A0A3B3IRJ8 | - | - |
| HDAC8-252 | ENST00000649518.1 | 2958 | 193aa | Nonsense mediated decay | - | A0A3B3IRJ8 | - | - |
| HDAC8-253 | ENST00000649543.1 | 1941 | 193aa | Nonsense mediated decay | - | A0A3B3IRJ8 | - | - |
| HDAC8-247 | ENST00000649097.1 | 1940 | 375aa | Nonsense mediated decay | - | A0A3B3IT04 | - | - |
| HDAC8-226 | ENST00000647974.1 | 1770 | 15aa | Nonsense mediated decay | - | A0A3B3ISG7 | - | CDS 5' incomplete, |
| HDAC8-237 | ENST00000648504.1 | 1756 | 286aa | Nonsense mediated decay | - | A0A3B3IT95 | - | CDS 5' incomplete, |
| HDAC8-240 | ENST00000648731.1 | 1716 | 167aa | Nonsense mediated decay | - | A0A3B3ITV2 | - | CDS 5' incomplete, |
| HDAC8-245 | ENST00000648939.1 | 1659 | 307aa | Nonsense mediated decay | - | A0A3B3ISS7 | - | - |
| HDAC8-241 | ENST00000648834.1 | 1484 | 307aa | Nonsense mediated decay | - | A0A3B3ISS7 | - | - |
| HDAC8-251 | ENST00000649274.1 | 1479 | 24aa | Nonsense mediated decay | - | A0A3B3IS08 | - | CDS 5' incomplete, |
| HDAC8-249 | ENST00000649181.1 | 1477 | 174aa | Nonsense mediated decay | - | A0A3B3IUD1 | - | - |
| HDAC8-257 | ENST00000650471.1 | 1323 | 137aa | Nonsense mediated decay | - | A0A3B3IU21 | - | - |
| HDAC8-242 | ENST00000648850.1 | 1308 | 10aa | Nonsense mediated decay | - | A0A3B3ISN6 | - | CDS 5' incomplete, |
| HDAC8-213 | ENST00000436675.6 | 963 | 185aa | Nonsense mediated decay | - | E7EW22 | - | TSL:5, |
| HDAC8-239 | ENST00000648711.1 | 851 | 182aa | Nonsense mediated decay | - | A0A3B3IU62 | - | CDS 5' incomplete, |
| HDAC8-220 | ENST00000647606.1 | 827 | 71aa | Nonsense mediated decay | - | A0A3B3ISB8 | - | CDS 5' incomplete, |
| HDAC8-210 | ENST00000412342.6 | 687 | 56aa | Nonsense mediated decay | - | F8WCG4 | - | TSL:1, |
| HDAC8-216 | ENST00000470998.2 | 333 | 16aa | Nonsense mediated decay | - | A0A3B3ISP0 | - | CDS 5' incomplete, TSL:3, |
| HDAC8-221 | ENST00000647641.1 | 3444 | No protein | Processed transcript | - | - | - | - |
| HDAC8-217 | ENST00000478743.2 | 1909 | No protein | Processed transcript | - | - | - | TSL:4, |
| HDAC8-258 | ENST00000650477.1 | 675 | No protein | Processed transcript | - | - | - | - |
| HDAC8-215 | ENST00000444609.1 | 607 | No protein | Processed transcript | - | - | - | TSL:3, |
| HDAC8-229 | ENST00000648101.1 | 592 | No protein | Processed transcript | - | - | - | - |
| HDAC8-223 | ENST00000647718.1 | 5369 | No protein | Retained intron | - | - | - | - |
| HDAC8-231 | ENST00000648158.1 | 2313 | No protein | Retained intron | - | - | - | - |
| HDAC8-243 | ENST00000648855.1 | 1741 | No protein | Retained intron | - | - | - | - |
| HDAC8-233 | ENST00000648285.1 | 1223 | No protein | Retained intron | - | - | - | - |
| HDAC8-218 | ENST00000486704.1 | 330 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HDAC8 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HDAC8 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
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Single gRNA lentivirus
Work flow
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