CRISPR-U™ Gene Knockout Cell Line
Strategy
ECHDC1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ECHDC1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ECHDC1 |
| Gene id | 55862 |
| Organism | Homo sapiens |
| Official full symbol | ethylmalonyl-CoA decarboxylase 1 |
| Gene type | protein-coding |
| Also known as | HEL-S-76, MMCD, dJ351K20.2 |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 14 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ECHDC1-222 | ENST00000531967.5 | 2585 | 307aa | Protein coding | CCDS47471 | Q9NTX5-1 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| ECHDC1-214 | ENST00000474289.6 | 2443 | 301aa | Protein coding | CCDS43504 | Q9NTX5-2 | - | TSL:5, GENCODE basic, APPRIS P2, |
| ECHDC1-210 | ENST00000454859.8 | 2339 | 301aa | Protein coding | CCDS43504 | Q9NTX5-2 | NM_001002030.2 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ECHDC1-209 | ENST00000454591.6 | 1515 | 226aa | Protein coding | CCDS47472 | Q9NTX5-3 | - | TSL:2, GENCODE basic, |
| ECHDC1-220 | ENST00000528402.5 | 1449 | 70aa | Protein coding | CCDS55054 | Q9NTX5-4 | - | TSL:1, GENCODE basic, |
| ECHDC1-203 | ENST00000368291.6 | 1354 | 145aa | Protein coding | CCDS34530 | Q9NTX5-5 | - | TSL:2, GENCODE basic, |
| ECHDC1-207 | ENST00000430841.6 | 1009 | 301aa | Protein coding | CCDS43504 | Q9NTX5-2 | - | TSL:5, GENCODE basic, APPRIS P2, |
| ECHDC1-202 | ENST00000368289.6 | 909 | 145aa | Protein coding | CCDS34530 | Q9NTX5-5 | - | TSL:5, GENCODE basic, |
| ECHDC1-208 | ENST00000436638.5 | 766 | 255aa | Protein coding | - | H0Y5L2 | - | CDS 5' and 3' incomplete, TSL:3, |
| ECHDC1-212 | ENST00000461239.2 | 617 | 34aa | Protein coding | - | H0YD54 | - | CDS 5' incomplete, TSL:5, |
| ECHDC1-218 | ENST00000525745.5 | 601 | 179aa | Protein coding | - | E9PLY6 | - | CDS 3' incomplete, TSL:5, |
| ECHDC1-223 | ENST00000534442.5 | 577 | 151aa | Protein coding | - | E9PJS8 | - | CDS 3' incomplete, TSL:5, |
| ECHDC1-221 | ENST00000531582.1 | 531 | 135aa | Protein coding | - | E9PRU6 | - | CDS 3' incomplete, TSL:4, |
| ECHDC1-211 | ENST00000460558.5 | 301 | 100aa | Protein coding | - | H0YCS9 | - | CDS 5' and 3' incomplete, TSL:5, |
| ECHDC1-205 | ENST00000368295.8 | 1966 | 125aa | Nonsense mediated decay | - | J3KP84 | - | TSL:5, |
| ECHDC1-216 | ENST00000479525.6 | 1368 | 125aa | Nonsense mediated decay | - | E9PPG7 | - | TSL:5, |
| ECHDC1-204 | ENST00000368292.10 | 854 | 139aa | Nonsense mediated decay | - | F2Z2D6 | - | TSL:5, |
| ECHDC1-206 | ENST00000417628.6 | 767 | 76aa | Nonsense mediated decay | - | H0Y525 | - | CDS 5' incomplete, TSL:5, |
| ECHDC1-219 | ENST00000526299.5 | 534 | 83aa | Nonsense mediated decay | - | E9PIU8 | - | TSL:5, |
| ECHDC1-217 | ENST00000488087.1 | 473 | No protein | Processed transcript | - | - | - | TSL:3, |
| ECHDC1-215 | ENST00000475319.1 | 1543 | No protein | Retained intron | - | - | - | TSL:2, |
| ECHDC1-201 | ENST00000368287.1 | 509 | No protein | Retained intron | - | - | - | TSL:2, |
| ECHDC1-213 | ENST00000474240.1 | 482 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ECHDC1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ECHDC1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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