CRISPR-U™ Gene Knockout Cell Line Strategy

ECHDC1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ECHDC1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ECHDC1
Gene id 55862
Organism Homo sapiens
Official full symbol ethylmalonyl-CoA decarboxylase 1
Gene type protein-coding
Also known as HEL-S-76, MMCD, dJ351K20.2
Genomic regions Chromosome 6
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ECHDC1-222 ENST00000531967.5 2585 307aa Protein coding CCDS47471 Q9NTX5-1 - TSL:2, GENCODE basic, APPRIS ALT2,
ECHDC1-214 ENST00000474289.6 2443 301aa Protein coding CCDS43504 Q9NTX5-2 - TSL:5, GENCODE basic, APPRIS P2,
ECHDC1-210 ENST00000454859.8 2339 301aa Protein coding CCDS43504 Q9NTX5-2 NM_001002030.2 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
ECHDC1-209 ENST00000454591.6 1515 226aa Protein coding CCDS47472 Q9NTX5-3 - TSL:2, GENCODE basic,
ECHDC1-220 ENST00000528402.5 1449 70aa Protein coding CCDS55054 Q9NTX5-4 - TSL:1, GENCODE basic,
ECHDC1-203 ENST00000368291.6 1354 145aa Protein coding CCDS34530 Q9NTX5-5 - TSL:2, GENCODE basic,
ECHDC1-207 ENST00000430841.6 1009 301aa Protein coding CCDS43504 Q9NTX5-2 - TSL:5, GENCODE basic, APPRIS P2,
ECHDC1-202 ENST00000368289.6 909 145aa Protein coding CCDS34530 Q9NTX5-5 - TSL:5, GENCODE basic,
ECHDC1-208 ENST00000436638.5 766 255aa Protein coding - H0Y5L2 - CDS 5' and 3' incomplete, TSL:3,
ECHDC1-212 ENST00000461239.2 617 34aa Protein coding - H0YD54 - CDS 5' incomplete, TSL:5,
ECHDC1-218 ENST00000525745.5 601 179aa Protein coding - E9PLY6 - CDS 3' incomplete, TSL:5,
ECHDC1-223 ENST00000534442.5 577 151aa Protein coding - E9PJS8 - CDS 3' incomplete, TSL:5,
ECHDC1-221 ENST00000531582.1 531 135aa Protein coding - E9PRU6 - CDS 3' incomplete, TSL:4,
ECHDC1-211 ENST00000460558.5 301 100aa Protein coding - H0YCS9 - CDS 5' and 3' incomplete, TSL:5,
ECHDC1-205 ENST00000368295.8 1966 125aa Nonsense mediated decay - J3KP84 - TSL:5,
ECHDC1-216 ENST00000479525.6 1368 125aa Nonsense mediated decay - E9PPG7 - TSL:5,
ECHDC1-204 ENST00000368292.10 854 139aa Nonsense mediated decay - F2Z2D6 - TSL:5,
ECHDC1-206 ENST00000417628.6 767 76aa Nonsense mediated decay - H0Y525 - CDS 5' incomplete, TSL:5,
ECHDC1-219 ENST00000526299.5 534 83aa Nonsense mediated decay - E9PIU8 - TSL:5,
ECHDC1-217 ENST00000488087.1 473 No protein Processed transcript - - - TSL:3,
ECHDC1-215 ENST00000475319.1 1543 No protein Retained intron - - - TSL:2,
ECHDC1-201 ENST00000368287.1 509 No protein Retained intron - - - TSL:2,
ECHDC1-213 ENST00000474240.1 482 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ECHDC1
This KO Strategy loading
Red Cotton™ Notes Gene ECHDC1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×