CRISPR-U™ Gene Knockout Cell Line
Strategy
PKN2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PKN2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PKN2 |
| Gene id | 5586 |
| Organism | Homo sapiens |
| Official full symbol | protein kinase N2 |
| Gene type | protein-coding |
| Also known as | PAK2, PRK2, PRKCL2, PRO2042, Pak-2, STK7 |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PKN2-203 | ENST00000370521.8 | 6070 | 984aa | Protein coding | CCDS714 | Q16513-1 | NM_006256.4 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| PKN2-202 | ENST00000370513.9 | 2890 | 936aa | Protein coding | CCDS81350 | Q16513-3 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PKN2-201 | ENST00000316005.11 | 2632 | 604aa | Protein coding | - | B1AL79 | - | TSL:5, GENCODE basic, |
| PKN2-204 | ENST00000436111.1 | 632 | 211aa | Protein coding | - | H0Y5V5 | - | CDS 5' and 3' incomplete, TSL:3, |
| PKN2-205 | ENST00000449189.1 | 342 | 65aa | Protein coding | - | H0Y429 | - | CDS 5' incomplete, TSL:3, |
| PKN2-206 | ENST00000495119.1 | 416 | No protein | Processed transcript | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PKN2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PKN2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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