CRISPR-U™ Gene Knockout Cell Line Strategy

PKN2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PKN2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PKN2
Gene id 5586
Organism Homo sapiens
Official full symbol protein kinase N2
Gene type protein-coding
Also known as PAK2, PRK2, PRKCL2, PRO2042, Pak-2, STK7
Genomic regions Chromosome 1
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PKN2-203 ENST00000370521.8 6070 984aa Protein coding CCDS714 Q16513-1 NM_006256.4 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
PKN2-202 ENST00000370513.9 2890 936aa Protein coding CCDS81350 Q16513-3 - TSL:1, GENCODE basic, APPRIS ALT2,
PKN2-201 ENST00000316005.11 2632 604aa Protein coding - B1AL79 - TSL:5, GENCODE basic,
PKN2-204 ENST00000436111.1 632 211aa Protein coding - H0Y5V5 - CDS 5' and 3' incomplete, TSL:3,
PKN2-205 ENST00000449189.1 342 65aa Protein coding - H0Y429 - CDS 5' incomplete, TSL:3,
PKN2-206 ENST00000495119.1 416 No protein Processed transcript - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PKN2
This KO Strategy loading
Red Cotton™ Notes Gene PKN2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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