CRISPR-U™ Gene Knockout Cell Line
Strategy
PKN1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PKN1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PKN1 |
| Gene id | 5585 |
| Organism | Homo sapiens |
| Official full symbol | protein kinase N1 |
| Gene type | protein-coding |
| Also known as | DBK, PAK-1, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1 |
| Summary | The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PKN1-201 | ENST00000242783.11 | 3120 | 942aa | Protein coding | CCDS42513 | Q16512-1 | NM_002741.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| PKN1-202 | ENST00000342216.8 | 2960 | 948aa | Protein coding | CCDS42514 | Q16512-2 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| PKN1-207 | ENST00000586557.5 | 899 | 255aa | Protein coding | - | K7EKY9 | - | CDS 3' incomplete, TSL:5, |
| PKN1-212 | ENST00000590097.5 | 848 | 263aa | Protein coding | - | K7EM57 | - | CDS 3' incomplete, TSL:3, |
| PKN1-215 | ENST00000592960.1 | 677 | 226aa | Protein coding | - | K7ES10 | - | CDS 5' and 3' incomplete, TSL:3, |
| PKN1-203 | ENST00000585619.5 | 582 | 14aa | Protein coding | - | K7EPK3 | - | CDS 3' incomplete, TSL:5, |
| PKN1-206 | ENST00000586237.1 | 529 | 151aa | Protein coding | - | K7EN76 | - | CDS 5' incomplete, TSL:5, |
| PKN1-213 | ENST00000591461.1 | 867 | 198aa | Nonsense mediated decay | - | K7EL10 | - | CDS 5' incomplete, TSL:3, |
| PKN1-210 | ENST00000587429.1 | 562 | No protein | Processed transcript | - | - | - | TSL:4, |
| PKN1-214 | ENST00000592794.1 | 322 | No protein | Processed transcript | - | - | - | TSL:4, |
| PKN1-204 | ENST00000585839.5 | 3518 | No protein | Retained intron | - | - | - | TSL:2, |
| PKN1-205 | ENST00000586039.1 | 686 | No protein | Retained intron | - | - | - | TSL:2, |
| PKN1-208 | ENST00000586900.1 | 550 | No protein | Retained intron | - | - | - | TSL:3, |
| PKN1-211 | ENST00000588200.1 | 548 | No protein | Retained intron | - | - | - | TSL:3, |
| PKN1-209 | ENST00000587215.1 | 363 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PKN1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PKN1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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