CRISPR-U™ Gene Knockout Cell Line Strategy

PKN1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PKN1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PKN1
Gene id 5585
Organism Homo sapiens
Official full symbol protein kinase N1
Gene type protein-coding
Also known as DBK, PAK-1, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1
Summary The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Genomic regions Chromosome 19
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PKN1-201 ENST00000242783.11 3120 942aa Protein coding CCDS42513 Q16512-1 NM_002741.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
PKN1-202 ENST00000342216.8 2960 948aa Protein coding CCDS42514 Q16512-2 - TSL:2, GENCODE basic, APPRIS ALT1,
PKN1-207 ENST00000586557.5 899 255aa Protein coding - K7EKY9 - CDS 3' incomplete, TSL:5,
PKN1-212 ENST00000590097.5 848 263aa Protein coding - K7EM57 - CDS 3' incomplete, TSL:3,
PKN1-215 ENST00000592960.1 677 226aa Protein coding - K7ES10 - CDS 5' and 3' incomplete, TSL:3,
PKN1-203 ENST00000585619.5 582 14aa Protein coding - K7EPK3 - CDS 3' incomplete, TSL:5,
PKN1-206 ENST00000586237.1 529 151aa Protein coding - K7EN76 - CDS 5' incomplete, TSL:5,
PKN1-213 ENST00000591461.1 867 198aa Nonsense mediated decay - K7EL10 - CDS 5' incomplete, TSL:3,
PKN1-210 ENST00000587429.1 562 No protein Processed transcript - - - TSL:4,
PKN1-214 ENST00000592794.1 322 No protein Processed transcript - - - TSL:4,
PKN1-204 ENST00000585839.5 3518 No protein Retained intron - - - TSL:2,
PKN1-205 ENST00000586039.1 686 No protein Retained intron - - - TSL:2,
PKN1-208 ENST00000586900.1 550 No protein Retained intron - - - TSL:3,
PKN1-211 ENST00000588200.1 548 No protein Retained intron - - - TSL:3,
PKN1-209 ENST00000587215.1 363 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PKN1
This KO Strategy loading
Red Cotton™ Notes Gene PKN1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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