CRISPR-U™ Gene Knockout Cell Line
Strategy
CISD1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CISD1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CISD1 |
| Gene id | 55847 |
| Organism | Homo sapiens |
| Official full symbol | CDGSH iron sulfur domain 1 |
| Gene type | protein-coding |
| Also known as | C10orf70, MDS029, ZCD1, mitoNEET |
| Summary | This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CISD1-201 | ENST00000333926.6 | 2375 | 108aa | Protein coding | CCDS7251 | A0A024QZN7 Q9NZ45 | NM_018464.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CISD1-203 | ENST00000488388.2 | 881 | No protein | Processed transcript | - | - | - | TSL:5, |
| CISD1-202 | ENST00000464703.5 | 698 | No protein | Processed transcript | - | - | - | TSL:5, |
| CISD1-204 | ENST00000489785.5 | 577 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CISD1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CISD1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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