CRISPR-U™ Gene Knockout Cell Line Strategy

CISD1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CISD1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CISD1
Gene id 55847
Organism Homo sapiens
Official full symbol CDGSH iron sulfur domain 1
Gene type protein-coding
Also known as C10orf70, MDS029, ZCD1, mitoNEET
Summary This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.
Genomic regions Chromosome 10
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CISD1-201 ENST00000333926.6 2375 108aa Protein coding CCDS7251 A0A024QZN7 Q9NZ45 NM_018464.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CISD1-203 ENST00000488388.2 881 No protein Processed transcript - - - TSL:5,
CISD1-202 ENST00000464703.5 698 No protein Processed transcript - - - TSL:5,
CISD1-204 ENST00000489785.5 577 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CISD1
This KO Strategy loading
Red Cotton™ Notes Gene CISD1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×