CRISPR-U™ Gene Knockout Cell Line
Strategy
BRK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BRK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BRK1 |
| Gene id | 55845 |
| Organism | Homo sapiens |
| Official full symbol | BRICK1 subunit of SCAR/WAVE actin nucleating complex |
| Gene type | protein-coding |
| Also known as | C3orf10, HSPC300, MDS027, hHBrk1 |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BRK1-201 | ENST00000530758.2 | 1150 | 75aa | Protein coding | CCDS54553 | Q8WUW1-1 | NM_018462.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BRK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BRK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: