CRISPR-U™ Gene Knockout Cell Line Strategy

BRK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BRK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BRK1
Gene id 55845
Organism Homo sapiens
Official full symbol BRICK1 subunit of SCAR/WAVE actin nucleating complex
Gene type protein-coding
Also known as C3orf10, HSPC300, MDS027, hHBrk1
Genomic regions Chromosome 3
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BRK1-201 ENST00000530758.2 1150 75aa Protein coding CCDS54553 Q8WUW1-1 NM_018462.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BRK1
This KO Strategy loading
Red Cotton™ Notes Gene BRK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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