CRISPR-U™ Gene Knockout Cell Line Strategy

PRKCD Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PRKCD Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRKCD
Gene id 5580
Organism Homo sapiens
Official full symbol protein kinase C delta
Gene type protein-coding
Also known as ALPS3, CVID9, MAY1, PKCD, nPKC-delta
Summary The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.
Genomic regions Chromosome 3
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRKCD-201 ENST00000330452.8 2833 676aa Protein coding CCDS2870 Q05655-1 NM_006254.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PRKCD-202 ENST00000394729.6 2810 676aa Protein coding CCDS2870 Q05655-1 - TSL:1, GENCODE basic, APPRIS P1,
PRKCD-210 ENST00000652449.1 2711 676aa Protein coding CCDS2870 Q05655-1 - GENCODE basic, APPRIS P1,
PRKCD-207 ENST00000650739.1 2531 676aa Protein coding CCDS2870 Q05655-1 - GENCODE basic, APPRIS P1,
PRKCD-211 ENST00000654719.1 2262 676aa Protein coding CCDS2870 Q05655-1 - GENCODE basic, APPRIS P1,
PRKCD-208 ENST00000650940.1 975 295aa Protein coding - A0A494C1T7 - CDS 3' incomplete,
PRKCD-203 ENST00000464818.1 777 223aa Protein coding - C9K0E3 - CDS 3' incomplete, TSL:5,
PRKCD-205 ENST00000478843.5 589 77aa Protein coding - C9JZU8 - CDS 3' incomplete, TSL:4,
PRKCD-206 ENST00000487897.5 562 125aa Protein coding - C9J9P1 - CDS 3' incomplete, TSL:4,
PRKCD-204 ENST00000477794.2 404 38aa Protein coding - A0A494C125 - CDS 3' incomplete, TSL:3,
PRKCD-209 ENST00000651505.1 2083 112aa Nonsense mediated decay - A0A494BZX2 - CDS 5' incomplete,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRKCD
This KO Strategy loading
Red Cotton™ Notes Gene PRKCD had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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