CRISPR-U™ Gene Knockout Cell Line
Strategy
PRKCD Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRKCD Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PRKCD |
| Gene id | 5580 |
| Organism | Homo sapiens |
| Official full symbol | protein kinase C delta |
| Gene type | protein-coding |
| Also known as | ALPS3, CVID9, MAY1, PKCD, nPKC-delta |
| Summary | The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PRKCD-201 | ENST00000330452.8 | 2833 | 676aa | Protein coding | CCDS2870 | Q05655-1 | NM_006254.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PRKCD-202 | ENST00000394729.6 | 2810 | 676aa | Protein coding | CCDS2870 | Q05655-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PRKCD-210 | ENST00000652449.1 | 2711 | 676aa | Protein coding | CCDS2870 | Q05655-1 | - | GENCODE basic, APPRIS P1, |
| PRKCD-207 | ENST00000650739.1 | 2531 | 676aa | Protein coding | CCDS2870 | Q05655-1 | - | GENCODE basic, APPRIS P1, |
| PRKCD-211 | ENST00000654719.1 | 2262 | 676aa | Protein coding | CCDS2870 | Q05655-1 | - | GENCODE basic, APPRIS P1, |
| PRKCD-208 | ENST00000650940.1 | 975 | 295aa | Protein coding | - | A0A494C1T7 | - | CDS 3' incomplete, |
| PRKCD-203 | ENST00000464818.1 | 777 | 223aa | Protein coding | - | C9K0E3 | - | CDS 3' incomplete, TSL:5, |
| PRKCD-205 | ENST00000478843.5 | 589 | 77aa | Protein coding | - | C9JZU8 | - | CDS 3' incomplete, TSL:4, |
| PRKCD-206 | ENST00000487897.5 | 562 | 125aa | Protein coding | - | C9J9P1 | - | CDS 3' incomplete, TSL:4, |
| PRKCD-204 | ENST00000477794.2 | 404 | 38aa | Protein coding | - | A0A494C125 | - | CDS 3' incomplete, TSL:3, |
| PRKCD-209 | ENST00000651505.1 | 2083 | 112aa | Nonsense mediated decay | - | A0A494BZX2 | - | CDS 5' incomplete, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PRKCD |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PRKCD had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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