CRISPR-U™ Gene Knockout Cell Line Strategy

AXL Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human AXL Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol AXL
Gene id 558
Organism Homo sapiens
Official full symbol AXL receptor tyrosine kinase
Gene type protein-coding
Also known as ARK, JTK11, Tyro7, UFO
Summary The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
AXL-201 ENST00000301178.9 4717 894aa Protein coding CCDS12575 P30530-1 NM_021913.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
AXL-202 ENST00000359092.7 3206 885aa Protein coding CCDS12574 P30530-2 - TSL:1, GENCODE basic, APPRIS ALT2,
AXL-203 ENST00000593513.1 2557 626aa Protein coding CCDS62677 M0R0W6 - TSL:1, GENCODE basic,
AXL-204 ENST00000594880.1 543 No protein Processed transcript - - - TSL:4,
AXL-205 ENST00000599659.5 1708 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene AXL
This KO Strategy loading
Red Cotton™ Notes Gene AXL had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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