CRISPR-U™ Gene Knockout Cell Line
Strategy
AXL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human AXL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | AXL |
| Gene id | 558 |
| Organism | Homo sapiens |
| Official full symbol | AXL receptor tyrosine kinase |
| Gene type | protein-coding |
| Also known as | ARK, JTK11, Tyro7, UFO |
| Summary | The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| AXL-201 | ENST00000301178.9 | 4717 | 894aa | Protein coding | CCDS12575 | P30530-1 | NM_021913.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| AXL-202 | ENST00000359092.7 | 3206 | 885aa | Protein coding | CCDS12574 | P30530-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| AXL-203 | ENST00000593513.1 | 2557 | 626aa | Protein coding | CCDS62677 | M0R0W6 | - | TSL:1, GENCODE basic, |
| AXL-204 | ENST00000594880.1 | 543 | No protein | Processed transcript | - | - | - | TSL:4, |
| AXL-205 | ENST00000599659.5 | 1708 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | AXL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene AXL had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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