CRISPR-U™ Gene Knockout Cell Line
Strategy
EXOC2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EXOC2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EXOC2 |
| Gene id | 55770 |
| Organism | Homo sapiens |
| Official full symbol | exocyst complex component 2 |
| Gene type | protein-coding |
| Also known as | SEC5, SEC5L1, Sec5p |
| Summary | The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EXOC2-201 | ENST00000230449.9 | 4456 | 924aa | Protein coding | CCDS34327 | A0A024QZT2 Q96KP1 | NM_018303.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EXOC2-202 | ENST00000443083.5 | 720 | 140aa | Protein coding | - | Q2MDF5 | - | CDS 3' incomplete, TSL:3, |
| EXOC2-203 | ENST00000475028.2 | 536 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EXOC2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EXOC2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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