CRISPR-U™ Gene Knockout Cell Line
Strategy
PRKAR2A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRKAR2A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PRKAR2A |
| Gene id | 5576 |
| Organism | Homo sapiens |
| Official full symbol | protein kinase cAMP-dependent type II regulatory subunit alpha |
| Gene type | protein-coding |
| Also known as | PKR2, PRKAR2 |
| Summary | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PRKAR2A-201 | ENST00000265563.13 | 6492 | 404aa | Protein coding | CCDS2778 | P13861-1 | NM_004157.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PRKAR2A-202 | ENST00000296446.12 | 3318 | 382aa | Protein coding | CCDS82771 | P13861-2 | - | TSL:1, GENCODE basic, |
| PRKAR2A-206 | ENST00000454963.5 | 1849 | 404aa | Protein coding | CCDS2778 | P13861-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| PRKAR2A-204 | ENST00000437821.2 | 627 | 200aa | Protein coding | - | H7C1L0 | - | CDS 5' incomplete, TSL:5, |
| PRKAR2A-205 | ENST00000438535.1 | 624 | 99aa | Protein coding | - | H7C4A9 | - | CDS 5' incomplete, TSL:3, |
| PRKAR2A-203 | ENST00000419216.1 | 581 | 114aa | Protein coding | - | C9J830 | - | CDS 3' incomplete, TSL:4, |
| PRKAR2A-207 | ENST00000457914.5 | 448 | 92aa | Protein coding | - | H7C330 | - | CDS 5' incomplete, TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PRKAR2A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PRKAR2A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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