CRISPR-U™ Gene Knockout Cell Line Strategy

PRKAR2A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PRKAR2A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRKAR2A
Gene id 5576
Organism Homo sapiens
Official full symbol protein kinase cAMP-dependent type II regulatory subunit alpha
Gene type protein-coding
Also known as PKR2, PRKAR2
Summary cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Genomic regions Chromosome 3
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRKAR2A-201 ENST00000265563.13 6492 404aa Protein coding CCDS2778 P13861-1 NM_004157.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PRKAR2A-202 ENST00000296446.12 3318 382aa Protein coding CCDS82771 P13861-2 - TSL:1, GENCODE basic,
PRKAR2A-206 ENST00000454963.5 1849 404aa Protein coding CCDS2778 P13861-1 - TSL:2, GENCODE basic, APPRIS P1,
PRKAR2A-204 ENST00000437821.2 627 200aa Protein coding - H7C1L0 - CDS 5' incomplete, TSL:5,
PRKAR2A-205 ENST00000438535.1 624 99aa Protein coding - H7C4A9 - CDS 5' incomplete, TSL:3,
PRKAR2A-203 ENST00000419216.1 581 114aa Protein coding - C9J830 - CDS 3' incomplete, TSL:4,
PRKAR2A-207 ENST00000457914.5 448 92aa Protein coding - H7C330 - CDS 5' incomplete, TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRKAR2A
This KO Strategy loading
Red Cotton™ Notes Gene PRKAR2A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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