CRISPR-U™ Gene Knockout Cell Line
Strategy
COA1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COA1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COA1 |
| Gene id | 55744 |
| Organism | Homo sapiens |
| Official full symbol | cytochrome c oxidase assembly factor 1 homolog |
| Gene type | protein-coding |
| Also known as | C7orf44, MITRAC15 |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COA1-203 | ENST00000395879.5 | 2448 | 146aa | Protein coding | CCDS5471 | A0A024RA60 Q9GZY4 | - | TSL:1, GENCODE basic, APPRIS P1, |
| COA1-202 | ENST00000310564.10 | 1085 | 146aa | Protein coding | CCDS5471 | A0A024RA60 Q9GZY4 | - | TSL:2, GENCODE basic, APPRIS P1, |
| COA1-201 | ENST00000223336.10 | 927 | 146aa | Protein coding | CCDS5471 | A0A024RA60 Q9GZY4 | - | TSL:2, GENCODE basic, APPRIS P1, |
| COA1-208 | ENST00000431651.5 | 720 | 88aa | Protein coding | - | C9J6J1 | - | CDS 3' incomplete, TSL:3, |
| COA1-205 | ENST00000415798.5 | 524 | 113aa | Protein coding | - | C9JA07 | - | CDS 3' incomplete, TSL:4, |
| COA1-209 | ENST00000438444.5 | 3223 | 146aa | Nonsense mediated decay | CCDS5471 | A0A024RA60 Q9GZY4 | - | TSL:2, |
| COA1-204 | ENST00000415076.6 | 1737 | 146aa | Nonsense mediated decay | CCDS5471 | A0A024RA60 Q9GZY4 | - | TSL:3, |
| COA1-210 | ENST00000446330.6 | 1520 | 146aa | Nonsense mediated decay | CCDS5471 | A0A024RA60 Q9GZY4 | - | TSL:2, |
| COA1-206 | ENST00000418140.5 | 1372 | 63aa | Nonsense mediated decay | CCDS83177 | F2Z2J3 | - | TSL:1, |
| COA1-211 | ENST00000446564.5 | 1177 | 146aa | Nonsense mediated decay | CCDS5471 | A0A024RA60 Q9GZY4 | - | TSL:2, |
| COA1-214 | ENST00000457939.1 | 406 | 63aa | Nonsense mediated decay | CCDS83177 | F2Z2J3 | - | TSL:3, |
| COA1-212 | ENST00000448704.5 | 1599 | 34aa | Nonsense mediated decay | - | F2Z2D1 | - | TSL:1, |
| COA1-213 | ENST00000451651.5 | 585 | 41aa | Nonsense mediated decay | - | F8WBS2 | - | TSL:3, |
| COA1-207 | ENST00000420441.1 | 547 | 34aa | Nonsense mediated decay | - | F2Z2D1 | - | TSL:2, |
| COA1-218 | ENST00000488813.1 | 1641 | No protein | Processed transcript | - | - | - | TSL:2, |
| COA1-215 | ENST00000459713.1 | 574 | No protein | Processed transcript | - | - | - | TSL:3, |
| COA1-216 | ENST00000470156.1 | 569 | No protein | Processed transcript | - | - | - | TSL:1, |
| COA1-217 | ENST00000478504.1 | 552 | No protein | Processed transcript | - | - | - | TSL:4, |
| COA1-219 | ENST00000490251.1 | 2135 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COA1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COA1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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