CRISPR-U™ Gene Knockout Cell Line Strategy

MAP7D1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAP7D1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAP7D1
Gene id 55700
Organism Homo sapiens
Official full symbol MAP7 domain containing 1
Gene type protein-coding
Also known as PARCC1, RPRC1
Genomic regions Chromosome 1
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAP7D1-201 ENST00000316156.8 3456 803aa Protein coding CCDS65493 Q3KQU3-2 - TSL:1, GENCODE basic, APPRIS ALT2,
MAP7D1-204 ENST00000373151.6 3324 841aa Protein coding CCDS30673 Q3KQU3-1 - TSL:1, GENCODE basic, APPRIS P4,
MAP7D1-203 ENST00000373150.8 3238 808aa Protein coding CCDS65492 Q3KQU3-4 - TSL:2, GENCODE basic, APPRIS ALT2,
MAP7D1-205 ENST00000429533.6 959 244aa Protein coding - C9JIR3 - CDS 3' incomplete, TSL:3,
MAP7D1-212 ENST00000530975.4 744 248aa Protein coding - H0YF21 - CDS 5' and 3' incomplete, TSL:5,
MAP7D1-211 ENST00000530729.1 567 140aa Protein coding - E9PLH3 - CDS 3' incomplete, TSL:3,
MAP7D1-202 ENST00000373148.5 1797 No protein Processed transcript - - - TSL:2,
MAP7D1-207 ENST00000474796.1 906 No protein Processed transcript - - - TSL:2,
MAP7D1-209 ENST00000487131.3 733 No protein Processed transcript - - - TSL:5,
MAP7D1-210 ENST00000527764.1 945 No protein Retained intron - - - TSL:2,
MAP7D1-208 ENST00000487114.1 890 No protein Retained intron - - - TSL:2,
MAP7D1-206 ENST00000462118.1 776 No protein Retained intron - - - TSL:3,
MAP7D1-213 ENST00000532131.1 771 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAP7D1
This KO Strategy loading
Red Cotton™ Notes Gene MAP7D1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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