CRISPR-U™ Gene Knockout Cell Line
Strategy
MAP7D1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAP7D1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAP7D1 |
| Gene id | 55700 |
| Organism | Homo sapiens |
| Official full symbol | MAP7 domain containing 1 |
| Gene type | protein-coding |
| Also known as | PARCC1, RPRC1 |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAP7D1-201 | ENST00000316156.8 | 3456 | 803aa | Protein coding | CCDS65493 | Q3KQU3-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| MAP7D1-204 | ENST00000373151.6 | 3324 | 841aa | Protein coding | CCDS30673 | Q3KQU3-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| MAP7D1-203 | ENST00000373150.8 | 3238 | 808aa | Protein coding | CCDS65492 | Q3KQU3-4 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| MAP7D1-205 | ENST00000429533.6 | 959 | 244aa | Protein coding | - | C9JIR3 | - | CDS 3' incomplete, TSL:3, |
| MAP7D1-212 | ENST00000530975.4 | 744 | 248aa | Protein coding | - | H0YF21 | - | CDS 5' and 3' incomplete, TSL:5, |
| MAP7D1-211 | ENST00000530729.1 | 567 | 140aa | Protein coding | - | E9PLH3 | - | CDS 3' incomplete, TSL:3, |
| MAP7D1-202 | ENST00000373148.5 | 1797 | No protein | Processed transcript | - | - | - | TSL:2, |
| MAP7D1-207 | ENST00000474796.1 | 906 | No protein | Processed transcript | - | - | - | TSL:2, |
| MAP7D1-209 | ENST00000487131.3 | 733 | No protein | Processed transcript | - | - | - | TSL:5, |
| MAP7D1-210 | ENST00000527764.1 | 945 | No protein | Retained intron | - | - | - | TSL:2, |
| MAP7D1-208 | ENST00000487114.1 | 890 | No protein | Retained intron | - | - | - | TSL:2, |
| MAP7D1-206 | ENST00000462118.1 | 776 | No protein | Retained intron | - | - | - | TSL:3, |
| MAP7D1-213 | ENST00000532131.1 | 771 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAP7D1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAP7D1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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