CRISPR-U™ Gene Knockout Cell Line Strategy

PRKACB Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PRKACB Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRKACB
Gene id 5567
Organism Homo sapiens
Official full symbol protein kinase cAMP-activated catalytic subunit beta
Gene type protein-coding
Also known as PKA C-beta, PKACB
Summary The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Genomic regions Chromosome 1
Strategy Summary
This gene has 17 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRKACB-206 ENST00000370689.6 4513 351aa Protein coding CCDS691 P22694-1 - TSL:1, GENCODE basic, APPRIS P1,
PRKACB-204 ENST00000370685.7 4481 398aa Protein coding CCDS693 P22694-2 NM_182948.4 TSL:1, GENCODE basic, MANE Select v0.92,
PRKACB-219 ENST00000614872.4 4306 357aa Protein coding CCDS72815 P22694-6 - TSL:1, GENCODE basic,
PRKACB-202 ENST00000370682.7 4288 355aa Protein coding CCDS55610 P22694-7 - TSL:1, GENCODE basic,
PRKACB-218 ENST00000610703.4 4240 339aa Protein coding CCDS72813 P22694-3 - TSL:2, GENCODE basic,
PRKACB-217 ENST00000610457.1 4206 338aa Protein coding CCDS72816 A0A087WVC4 - TSL:2, GENCODE basic,
PRKACB-208 ENST00000394839.6 4200 321aa Protein coding CCDS55611 P22694-10 - TSL:2, GENCODE basic,
PRKACB-201 ENST00000370680.5 1914 264aa Protein coding CCDS72814 B1APG3 - TSL:1, GENCODE basic,
PRKACB-205 ENST00000370688.7 1905 257aa Protein coding CCDS692 P22694-8 - TSL:1, GENCODE basic,
PRKACB-213 ENST00000446538.5 1112 358aa Protein coding CCDS55609 P22694-9 - TSL:2, GENCODE basic,
PRKACB-203 ENST00000370684.5 869 245aa Protein coding CCDS72812 B1APF9 - TSL:5, GENCODE basic,
PRKACB-207 ENST00000394838.6 4294 357aa Protein coding - A0A0A0MS54 - TSL:5, GENCODE basic,
PRKACB-214 ENST00000450730.5 944 206aa Protein coding - B1APG0 - CDS 3' incomplete, TSL:3,
PRKACB-209 ENST00000413538.5 730 209aa Protein coding - B1APG2 - CDS 3' incomplete, TSL:5,
PRKACB-210 ENST00000417530.5 686 197aa Protein coding - B1APG1 - CDS 3' incomplete, TSL:2,
PRKACB-211 ENST00000432111.5 662 214aa Protein coding - B1APF8 - CDS 3' incomplete, TSL:5,
PRKACB-212 ENST00000436133.5 648 186aa Protein coding - B1APF7 - CDS 3' incomplete, TSL:3,
PRKACB-216 ENST00000470673.5 752 No protein Processed transcript - - - TSL:3,
PRKACB-215 ENST00000467507.1 543 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRKACB
This KO Strategy loading
Red Cotton™ Notes Gene PRKACB had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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