CRISPR-U™ Gene Knockout Cell Line Strategy

PRCC Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRCC Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRCC
Gene id 5546
Organism Homo sapiens
Official full symbol proline rich mitotic checkpoint control factor
Gene type protein-coding
Also known as RCCP1, TPRC
Summary This gene encodes a protein that may play a role in pre-mRNA splicing. Chromosomal translocations (X;1)(p11;q21) that result in fusion of this gene to TFE3 (GeneID 7030) have been associated with papillary renal cell carcinoma. A PRCC-TFE3 fusion protein is expressed in affected carcinomas and is likely associated with altered gene transactivation. This fusion protein has also been associated with disruption of the cell cycle.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRCC-201 ENST00000271526.9 2068 491aa Protein coding CCDS1157 A0A0S2Z456 Q92733 NM_005973.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PRCC-202 ENST00000454659.1 1084 257aa Protein coding - H0Y679 - CDS 5' incomplete, TSL:3,
PRCC-207 ENST00000526188.5 634 198aa Protein coding - H0YE12 - CDS 5' incomplete, TSL:2,
PRCC-206 ENST00000491853.5 833 No protein Processed transcript - - - TSL:3,
PRCC-205 ENST00000473387.1 452 No protein Processed transcript - - - TSL:2,
PRCC-204 ENST00000469071.1 1969 No protein Retained intron - - - TSL:2,
PRCC-203 ENST00000459707.1 557 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRCC
This KO Strategy loading
Red Cotton™ Notes Gene PRCC had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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