CRISPR-U™ Gene Knockout Cell Line
Strategy
PPP5C Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP5C Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPP5C |
| Gene id | 5536 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase 5 catalytic subunit |
| Gene type | protein-coding |
| Also known as | PP5, PPP5, PPT |
| Summary | This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPP5C-201 | ENST00000012443.9 | 2139 | 499aa | Protein coding | CCDS12684 | A0A024R0Q7 P53041 | NM_006247.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPP5C-202 | ENST00000391919.1 | 1929 | 371aa | Protein coding | - | A8MU39 | - | TSL:5, GENCODE basic, |
| PPP5C-205 | ENST00000478046.5 | 1886 | 485aa | Nonsense mediated decay | - | H0YDU8 | - | CDS 5' incomplete, TSL:1, |
| PPP5C-207 | ENST00000487483.6 | 4675 | No protein | Retained intron | - | - | - | TSL:5, |
| PPP5C-206 | ENST00000486994.5 | 2209 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP5C-214 | ENST00000532058.1 | 1970 | No protein | Retained intron | - | - | - | TSL:4, |
| PPP5C-211 | ENST00000525507.5 | 1300 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP5C-203 | ENST00000467502.6 | 1235 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP5C-213 | ENST00000527623.1 | 1215 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP5C-208 | ENST00000491003.5 | 878 | No protein | Retained intron | - | - | - | TSL:3, |
| PPP5C-209 | ENST00000492109.6 | 848 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP5C-215 | ENST00000595055.1 | 556 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP5C-212 | ENST00000527193.1 | 548 | No protein | Retained intron | - | - | - | TSL:3, |
| PPP5C-210 | ENST00000493347.5 | 431 | No protein | Retained intron | - | - | - | TSL:3, |
| PPP5C-204 | ENST00000467902.2 | 399 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPP5C |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPP5C had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
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$599
Single gRNA lentivirus
Work flow
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