CRISPR-U™ Gene Knockout Cell Line Strategy

PPP5C Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPP5C Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPP5C
Gene id 5536
Organism Homo sapiens
Official full symbol protein phosphatase 5 catalytic subunit
Gene type protein-coding
Also known as PP5, PPP5, PPT
Summary This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 19
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPP5C-201 ENST00000012443.9 2139 499aa Protein coding CCDS12684 A0A024R0Q7 P53041 NM_006247.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPP5C-202 ENST00000391919.1 1929 371aa Protein coding - A8MU39 - TSL:5, GENCODE basic,
PPP5C-205 ENST00000478046.5 1886 485aa Nonsense mediated decay - H0YDU8 - CDS 5' incomplete, TSL:1,
PPP5C-207 ENST00000487483.6 4675 No protein Retained intron - - - TSL:5,
PPP5C-206 ENST00000486994.5 2209 No protein Retained intron - - - TSL:2,
PPP5C-214 ENST00000532058.1 1970 No protein Retained intron - - - TSL:4,
PPP5C-211 ENST00000525507.5 1300 No protein Retained intron - - - TSL:2,
PPP5C-203 ENST00000467502.6 1235 No protein Retained intron - - - TSL:2,
PPP5C-213 ENST00000527623.1 1215 No protein Retained intron - - - TSL:2,
PPP5C-208 ENST00000491003.5 878 No protein Retained intron - - - TSL:3,
PPP5C-209 ENST00000492109.6 848 No protein Retained intron - - - TSL:2,
PPP5C-215 ENST00000595055.1 556 No protein Retained intron - - - TSL:2,
PPP5C-212 ENST00000527193.1 548 No protein Retained intron - - - TSL:3,
PPP5C-210 ENST00000493347.5 431 No protein Retained intron - - - TSL:3,
PPP5C-204 ENST00000467902.2 399 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPP5C
This KO Strategy loading
Red Cotton™ Notes Gene PPP5C had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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