CRISPR-U™ Gene Knockout Cell Line Strategy

LIN7C Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LIN7C Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LIN7C
Gene id 55327
Organism Homo sapiens
Official full symbol lin-7 homolog C, crumbs cell polarity complex component
Gene type protein-coding
Also known as LIN-7-C, LIN-7C, MALS-3, MALS3, VELI3
Genomic regions Chromosome 11
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LIN7C-201 ENST00000278193.7 4842 197aa Protein coding CCDS7864 Q9NUP9 NM_018362.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LIN7C-202 ENST00000524596.1 4290 173aa Protein coding - G3V1D4 - TSL:1, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LIN7C
This KO Strategy loading
Red Cotton™ Notes Gene LIN7C had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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