CRISPR-U™ Gene Knockout Cell Line
Strategy
PPP2R1B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP2R1B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPP2R1B |
| Gene id | 5519 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase 2 scaffold subunit Abeta |
| Gene type | protein-coding |
| Also known as | PP2A-Abeta, PR65B |
| Summary | This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPP2R1B-206 | ENST00000527614.6 | 5553 | 601aa | Protein coding | CCDS8349 | P30154-1 | NM_002716.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PPP2R1B-201 | ENST00000311129.9 | 2082 | 667aa | Protein coding | CCDS8348 | P30154-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PPP2R1B-202 | ENST00000341980.10 | 1953 | 556aa | Protein coding | CCDS53708 | P30154-4 | - | TSL:2, GENCODE basic, |
| PPP2R1B-204 | ENST00000426998.6 | 1888 | 603aa | Protein coding | CCDS53706 | P30154-3 | - | TSL:2, GENCODE basic, |
| PPP2R1B-203 | ENST00000393055.6 | 1525 | 474aa | Protein coding | CCDS53707 | P30154-5 | - | TSL:2, GENCODE basic, |
| PPP2R1B-210 | ENST00000531890.1 | 670 | 224aa | Protein coding | - | H0YDG7 | - | CDS 5' and 3' incomplete, TSL:5, |
| PPP2R1B-209 | ENST00000531373.1 | 553 | 119aa | Protein coding | - | E9PNM7 | - | CDS 3' incomplete, TSL:4, |
| PPP2R1B-212 | ENST00000534521.5 | 2294 | 135aa | Nonsense mediated decay | - | E9PHZ6 | - | TSL:2, |
| PPP2R1B-211 | ENST00000534500.5 | 582 | 83aa | Nonsense mediated decay | - | E9PPI5 | - | TSL:2, |
| PPP2R1B-208 | ENST00000530787.1 | 131 | No protein | Processed transcript | - | - | - | TSL:1, |
| PPP2R1B-205 | ENST00000526287.1 | 101 | No protein | Processed transcript | - | - | - | TSL:1, |
| PPP2R1B-207 | ENST00000529672.1 | 874 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPP2R1B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPP2R1B had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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