CRISPR-U™ Gene Knockout Cell Line Strategy

PPP2R1B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPP2R1B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPP2R1B
Gene id 5519
Organism Homo sapiens
Official full symbol protein phosphatase 2 scaffold subunit Abeta
Gene type protein-coding
Also known as PP2A-Abeta, PR65B
Summary This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described.
Genomic regions Chromosome 11
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPP2R1B-206 ENST00000527614.6 5553 601aa Protein coding CCDS8349 P30154-1 NM_002716.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PPP2R1B-201 ENST00000311129.9 2082 667aa Protein coding CCDS8348 P30154-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PPP2R1B-202 ENST00000341980.10 1953 556aa Protein coding CCDS53708 P30154-4 - TSL:2, GENCODE basic,
PPP2R1B-204 ENST00000426998.6 1888 603aa Protein coding CCDS53706 P30154-3 - TSL:2, GENCODE basic,
PPP2R1B-203 ENST00000393055.6 1525 474aa Protein coding CCDS53707 P30154-5 - TSL:2, GENCODE basic,
PPP2R1B-210 ENST00000531890.1 670 224aa Protein coding - H0YDG7 - CDS 5' and 3' incomplete, TSL:5,
PPP2R1B-209 ENST00000531373.1 553 119aa Protein coding - E9PNM7 - CDS 3' incomplete, TSL:4,
PPP2R1B-212 ENST00000534521.5 2294 135aa Nonsense mediated decay - E9PHZ6 - TSL:2,
PPP2R1B-211 ENST00000534500.5 582 83aa Nonsense mediated decay - E9PPI5 - TSL:2,
PPP2R1B-208 ENST00000530787.1 131 No protein Processed transcript - - - TSL:1,
PPP2R1B-205 ENST00000526287.1 101 No protein Processed transcript - - - TSL:1,
PPP2R1B-207 ENST00000529672.1 874 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPP2R1B
This KO Strategy loading
Red Cotton™ Notes Gene PPP2R1B had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×