CRISPR-U™ Gene Knockout Cell Line Strategy

ATG2B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATG2B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATG2B
Gene id 55102
Organism Homo sapiens
Official full symbol autophagy related 2B
Gene type protein-coding
Also known as C14orf103
Summary This gene encodes a protein required for autophagy. The encoded protein is involved in autophagosome formation. A germline duplication of a region that includes this gene is associated with predisposition to myeloid malignancies.
Genomic regions Chromosome 14
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATG2B-202 ENST00000359933.6 13162 2078aa Protein coding CCDS9944 Q96BY7 NM_018036.7 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
ATG2B-201 ENST00000261834.9 1920 No protein Processed transcript - - - TSL:2,
ATG2B-203 ENST00000473234.1 550 No protein Processed transcript - - - TSL:4,
ATG2B-204 ENST00000488421.1 522 No protein Processed transcript - - - TSL:4,
ATG2B-206 ENST00000554151.1 333 No protein Processed transcript - - - TSL:3,
ATG2B-207 ENST00000555263.1 492 No protein Retained intron - - - TSL:2,
ATG2B-205 ENST00000553643.1 482 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATG2B
This KO Strategy loading
Red Cotton™ Notes Gene ATG2B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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