CRISPR-U™ Gene Knockout Cell Line
Strategy
ATG2B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATG2B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATG2B |
| Gene id | 55102 |
| Organism | Homo sapiens |
| Official full symbol | autophagy related 2B |
| Gene type | protein-coding |
| Also known as | C14orf103 |
| Summary | This gene encodes a protein required for autophagy. The encoded protein is involved in autophagosome formation. A germline duplication of a region that includes this gene is associated with predisposition to myeloid malignancies. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATG2B-202 | ENST00000359933.6 | 13162 | 2078aa | Protein coding | CCDS9944 | Q96BY7 | NM_018036.7 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ATG2B-201 | ENST00000261834.9 | 1920 | No protein | Processed transcript | - | - | - | TSL:2, |
| ATG2B-203 | ENST00000473234.1 | 550 | No protein | Processed transcript | - | - | - | TSL:4, |
| ATG2B-204 | ENST00000488421.1 | 522 | No protein | Processed transcript | - | - | - | TSL:4, |
| ATG2B-206 | ENST00000554151.1 | 333 | No protein | Processed transcript | - | - | - | TSL:3, |
| ATG2B-207 | ENST00000555263.1 | 492 | No protein | Retained intron | - | - | - | TSL:2, |
| ATG2B-205 | ENST00000553643.1 | 482 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATG2B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATG2B had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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