CRISPR-U™ Gene Knockout Cell Line Strategy

PPP1CC Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP1CC Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPP1CC
Gene id 5501
Organism Homo sapiens
Official full symbol protein phosphatase 1 catalytic subunit gamma
Gene type protein-coding
Also known as PP-1G, PP1C, PPP1G
Summary The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPP1CC-201 ENST00000335007.10 2552 323aa Protein coding CCDS9150 P36873-1 NM_002710.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPP1CC-202 ENST00000340766.9 1472 337aa Protein coding CCDS58279 P36873-2 - TSL:2, GENCODE basic,
PPP1CC-204 ENST00000546933.5 2154 332aa Protein coding - F8W0W8 - TSL:3, GENCODE basic,
PPP1CC-206 ENST00000550991.5 1882 304aa Protein coding - F8VYE8 - TSL:2, GENCODE basic,
PPP1CC-208 ENST00000551676.5 1581 270aa Protein coding - F8VR82 - TSL:2, GENCODE basic,
PPP1CC-207 ENST00000551582.1 563 83aa Protein coding - F8W1A0 - CDS 5' incomplete, TSL:4,
PPP1CC-211 ENST00000620030.1 582 140aa Non stop decay - A0A087WYY5 - TSL:2,
PPP1CC-205 ENST00000550261.5 747 54aa Nonsense mediated decay - F8W0V8 - CDS 5' incomplete, TSL:5,
PPP1CC-209 ENST00000551690.1 603 No protein Processed transcript - - - TSL:3,
PPP1CC-210 ENST00000553024.1 2797 No protein Retained intron - - - TSL:2,
PPP1CC-203 ENST00000546904.1 1133 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPP1CC
This KO Strategy loading
Red Cotton™ Notes Gene PPP1CC had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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