CRISPR-U™ Gene Knockout Cell Line
Strategy
PPP1CC Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPP1CC Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPP1CC |
| Gene id | 5501 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase 1 catalytic subunit gamma |
| Gene type | protein-coding |
| Also known as | PP-1G, PP1C, PPP1G |
| Summary | The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPP1CC-201 | ENST00000335007.10 | 2552 | 323aa | Protein coding | CCDS9150 | P36873-1 | NM_002710.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPP1CC-202 | ENST00000340766.9 | 1472 | 337aa | Protein coding | CCDS58279 | P36873-2 | - | TSL:2, GENCODE basic, |
| PPP1CC-204 | ENST00000546933.5 | 2154 | 332aa | Protein coding | - | F8W0W8 | - | TSL:3, GENCODE basic, |
| PPP1CC-206 | ENST00000550991.5 | 1882 | 304aa | Protein coding | - | F8VYE8 | - | TSL:2, GENCODE basic, |
| PPP1CC-208 | ENST00000551676.5 | 1581 | 270aa | Protein coding | - | F8VR82 | - | TSL:2, GENCODE basic, |
| PPP1CC-207 | ENST00000551582.1 | 563 | 83aa | Protein coding | - | F8W1A0 | - | CDS 5' incomplete, TSL:4, |
| PPP1CC-211 | ENST00000620030.1 | 582 | 140aa | Non stop decay | - | A0A087WYY5 | - | TSL:2, |
| PPP1CC-205 | ENST00000550261.5 | 747 | 54aa | Nonsense mediated decay | - | F8W0V8 | - | CDS 5' incomplete, TSL:5, |
| PPP1CC-209 | ENST00000551690.1 | 603 | No protein | Processed transcript | - | - | - | TSL:3, |
| PPP1CC-210 | ENST00000553024.1 | 2797 | No protein | Retained intron | - | - | - | TSL:2, |
| PPP1CC-203 | ENST00000546904.1 | 1133 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPP1CC |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPP1CC had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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