CRISPR-U™ Gene Knockout Cell Line
Strategy
LAMTOR1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LAMTOR1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LAMTOR1 |
| Gene id | 55004 |
| Organism | Homo sapiens |
| Official full symbol | late endosomal/lysosomal adaptor, MAPK and MTOR activator 1 |
| Gene type | protein-coding |
| Also known as | C11orf59, PDRO, Ragulator1, p18, p27RF-Rho |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LAMTOR1-201 | ENST00000278671.10 | 1089 | 161aa | Protein coding | CCDS8209 | Q6IAA8 | NM_017907.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LAMTOR1-204 | ENST00000538404.1 | 1346 | 160aa | Protein coding | - | F5H3Y3 | - | TSL:2, GENCODE basic, |
| LAMTOR1-208 | ENST00000545249.5 | 965 | 142aa | Protein coding | - | F5GX19 | - | TSL:3, GENCODE basic, |
| LAMTOR1-207 | ENST00000544594.5 | 849 | 79aa | Protein coding | - | H0YFI1 | - | CDS 5' incomplete, TSL:3, |
| LAMTOR1-202 | ENST00000535107.5 | 619 | 99aa | Protein coding | - | F5H479 | - | TSL:3, GENCODE basic, |
| LAMTOR1-203 | ENST00000535872.1 | 899 | 76aa | Nonsense mediated decay | - | F5H267 | - | TSL:5, |
| LAMTOR1-205 | ENST00000539797.5 | 535 | No protein | Processed transcript | - | - | - | TSL:5, |
| LAMTOR1-206 | ENST00000541403.1 | 566 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LAMTOR1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LAMTOR1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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