CRISPR-U™ Gene Knockout Cell Line
Strategy
HCFC1R1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HCFC1R1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HCFC1R1 |
| Gene id | 54985 |
| Organism | Homo sapiens |
| Official full symbol | host cell factor C1 regulator 1 |
| Gene type | protein-coding |
| Also known as | HPIP |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HCFC1R1-207 | ENST00000574980.5 | 845 | 138aa | Protein coding | CCDS10490 | Q9NWW0-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| HCFC1R1-202 | ENST00000354679.3 | 765 | 136aa | Protein coding | CCDS73815 | J3KNY1 | - | TSL:2, GENCODE basic, |
| HCFC1R1-201 | ENST00000248089.8 | 656 | 138aa | Protein coding | CCDS10490 | Q9NWW0-1 | NM_017885.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HCFC1R1-206 | ENST00000574151.5 | 618 | 119aa | Protein coding | CCDS32375 | Q9NWW0-2 | - | TSL:1, GENCODE basic, |
| HCFC1R1-203 | ENST00000572355.5 | 610 | 98aa | Protein coding | CCDS76810 | I3L1N5 | - | TSL:3, GENCODE basic, |
| HCFC1R1-205 | ENST00000573842.1 | 495 | 120aa | Protein coding | - | I3L174 | - | CDS 3' incomplete, TSL:2, |
| HCFC1R1-208 | ENST00000575214.1 | 1365 | No protein | Retained intron | - | - | - | TSL:2, |
| HCFC1R1-209 | ENST00000576921.1 | 1199 | No protein | Retained intron | - | - | - | TSL:1, |
| HCFC1R1-204 | ENST00000573095.1 | 828 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HCFC1R1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HCFC1R1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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