CRISPR-U™ Gene Knockout Cell Line Strategy

PPM1G Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPM1G Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPM1G
Gene id 5496
Organism Homo sapiens
Official full symbol protein phosphatase, Mg2+/Mn2+ dependent 1G
Gene type protein-coding
Also known as PP2CG, PP2CGAMMA, PPP2CG
Summary The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.
Genomic regions Chromosome 2
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPM1G-201 ENST00000344034.5 2210 546aa Protein coding CCDS1752 O15355 Q6IAU5 NM_177983.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPM1G-202 ENST00000472077.1 3752 No protein Retained intron - - - TSL:2,
PPM1G-203 ENST00000484925.1 836 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPM1G
This KO Strategy loading
Red Cotton™ Notes Gene PPM1G had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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