CRISPR-U™ Gene Knockout Cell Line
Strategy
PPM1G Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPM1G Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPM1G |
| Gene id | 5496 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase, Mg2+/Mn2+ dependent 1G |
| Gene type | protein-coding |
| Also known as | PP2CG, PP2CGAMMA, PPP2CG |
| Summary | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPM1G-201 | ENST00000344034.5 | 2210 | 546aa | Protein coding | CCDS1752 | O15355 Q6IAU5 | NM_177983.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPM1G-202 | ENST00000472077.1 | 3752 | No protein | Retained intron | - | - | - | TSL:2, |
| PPM1G-203 | ENST00000484925.1 | 836 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPM1G |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPM1G had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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