CRISPR-U™ Gene Knockout Cell Line
Strategy
PPM1A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPM1A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPM1A |
| Gene id | 5494 |
| Organism | Homo sapiens |
| Official full symbol | protein phosphatase, Mg2+/Mn2+ dependent 1A |
| Gene type | protein-coding |
| Also known as | PP2C-ALPHA, PP2CA, PP2Calpha |
| Summary | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPM1A-203 | ENST00000395076.9 | 8231 | 382aa | Protein coding | CCDS9744 | P35813-1 | NM_021003.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPM1A-202 | ENST00000325658.3 | 4125 | 324aa | Protein coding | CCDS9745 | P35813-2 | - | TSL:1, GENCODE basic, |
| PPM1A-201 | ENST00000325642.7 | 1713 | 455aa | Protein coding | CCDS45120 | P35813-3 | - | TSL:2, GENCODE basic, |
| PPM1A-204 | ENST00000525399.2 | 854 | 187aa | Protein coding | - | E9PKB5 | - | CDS 3' incomplete, TSL:3, |
| PPM1A-207 | ENST00000531937.1 | 569 | 148aa | Protein coding | - | E9PJN3 | - | CDS 3' incomplete, TSL:4, |
| PPM1A-205 | ENST00000528241.2 | 564 | 128aa | Protein coding | - | E9PL75 | - | CDS 3' incomplete, TSL:5, |
| PPM1A-208 | ENST00000532036.2 | 1561 | 164aa | Nonsense mediated decay | - | E9PP44 | - | TSL:5, |
| PPM1A-206 | ENST00000531143.6 | 1546 | 93aa | Nonsense mediated decay | - | E9PNE1 | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPM1A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPM1A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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