CRISPR-U™ Gene Knockout Cell Line Strategy

PPM1A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPM1A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPM1A
Gene id 5494
Organism Homo sapiens
Official full symbol protein phosphatase, Mg2+/Mn2+ dependent 1A
Gene type protein-coding
Also known as PP2C-ALPHA, PP2CA, PP2Calpha
Summary The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Genomic regions Chromosome 14
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPM1A-203 ENST00000395076.9 8231 382aa Protein coding CCDS9744 P35813-1 NM_021003.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPM1A-202 ENST00000325658.3 4125 324aa Protein coding CCDS9745 P35813-2 - TSL:1, GENCODE basic,
PPM1A-201 ENST00000325642.7 1713 455aa Protein coding CCDS45120 P35813-3 - TSL:2, GENCODE basic,
PPM1A-204 ENST00000525399.2 854 187aa Protein coding - E9PKB5 - CDS 3' incomplete, TSL:3,
PPM1A-207 ENST00000531937.1 569 148aa Protein coding - E9PJN3 - CDS 3' incomplete, TSL:4,
PPM1A-205 ENST00000528241.2 564 128aa Protein coding - E9PL75 - CDS 3' incomplete, TSL:5,
PPM1A-208 ENST00000532036.2 1561 164aa Nonsense mediated decay - E9PP44 - TSL:5,
PPM1A-206 ENST00000531143.6 1546 93aa Nonsense mediated decay - E9PNE1 - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPM1A
This KO Strategy loading
Red Cotton™ Notes Gene PPM1A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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