CRISPR-U™ Gene Knockout Cell Line Strategy

NCAPG2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NCAPG2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NCAPG2
Gene id 54892
Organism Homo sapiens
Official full symbol non-SMC condensin II complex subunit G2
Gene type protein-coding
Also known as 3KS, CAP-G2, CAPG2, LUZP5, MTB, hCAP-G2
Summary This gene encodes a protein that belongs to the Condensin2nSMC family of proteins. The encoded protein is a regulatory subunit of the condensin II complex which, along with the condensin I complex, plays a role in chromosome assembly and segregation during mitosis. A similar protein in mouse is required for early development of the embryo. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 7
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NCAPG2-202 ENST00000409339.3 5253 1156aa Protein coding CCDS64816 Q86XI2-2 - TSL:1, GENCODE basic, APPRIS ALT2,
NCAPG2-201 ENST00000356309.8 4049 1143aa Protein coding CCDS43686 Q86XI2-1 NM_017760.7 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
NCAPG2-203 ENST00000409423.5 3957 1143aa Protein coding CCDS43686 Q86XI2-1 - TSL:2, GENCODE basic, APPRIS P4,
NCAPG2-205 ENST00000441982.5 2926 898aa Protein coding - H0Y6U5 - CDS 5' incomplete, TSL:2,
NCAPG2-204 ENST00000432615.5 4134 389aa Nonsense mediated decay - F8WE06 - TSL:5,
NCAPG2-211 ENST00000621338.1 597 No protein Processed transcript - - - TSL:3,
NCAPG2-208 ENST00000474940.5 583 No protein Processed transcript - - - TSL:4,
NCAPG2-210 ENST00000491792.5 581 No protein Processed transcript - - - TSL:3,
NCAPG2-209 ENST00000475918.5 557 No protein Processed transcript - - - TSL:4,
NCAPG2-207 ENST00000472591.2 554 No protein Processed transcript - - - TSL:4,
NCAPG2-206 ENST00000467785.5 3276 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NCAPG2
This KO Strategy loading
Red Cotton™ Notes Gene NCAPG2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×