CRISPR-U™ Gene Knockout Cell Line
Strategy
NCAPG2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NCAPG2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NCAPG2 |
| Gene id | 54892 |
| Organism | Homo sapiens |
| Official full symbol | non-SMC condensin II complex subunit G2 |
| Gene type | protein-coding |
| Also known as | 3KS, CAP-G2, CAPG2, LUZP5, MTB, hCAP-G2 |
| Summary | This gene encodes a protein that belongs to the Condensin2nSMC family of proteins. The encoded protein is a regulatory subunit of the condensin II complex which, along with the condensin I complex, plays a role in chromosome assembly and segregation during mitosis. A similar protein in mouse is required for early development of the embryo. Alternate splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NCAPG2-202 | ENST00000409339.3 | 5253 | 1156aa | Protein coding | CCDS64816 | Q86XI2-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| NCAPG2-201 | ENST00000356309.8 | 4049 | 1143aa | Protein coding | CCDS43686 | Q86XI2-1 | NM_017760.7 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| NCAPG2-203 | ENST00000409423.5 | 3957 | 1143aa | Protein coding | CCDS43686 | Q86XI2-1 | - | TSL:2, GENCODE basic, APPRIS P4, |
| NCAPG2-205 | ENST00000441982.5 | 2926 | 898aa | Protein coding | - | H0Y6U5 | - | CDS 5' incomplete, TSL:2, |
| NCAPG2-204 | ENST00000432615.5 | 4134 | 389aa | Nonsense mediated decay | - | F8WE06 | - | TSL:5, |
| NCAPG2-211 | ENST00000621338.1 | 597 | No protein | Processed transcript | - | - | - | TSL:3, |
| NCAPG2-208 | ENST00000474940.5 | 583 | No protein | Processed transcript | - | - | - | TSL:4, |
| NCAPG2-210 | ENST00000491792.5 | 581 | No protein | Processed transcript | - | - | - | TSL:3, |
| NCAPG2-209 | ENST00000475918.5 | 557 | No protein | Processed transcript | - | - | - | TSL:4, |
| NCAPG2-207 | ENST00000472591.2 | 554 | No protein | Processed transcript | - | - | - | TSL:4, |
| NCAPG2-206 | ENST00000467785.5 | 3276 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NCAPG2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NCAPG2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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