CRISPR-U™ Gene Knockout Cell Line Strategy

NSUN2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NSUN2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NSUN2
Gene id 54888
Organism Homo sapiens
Official full symbol NOP2/Sun RNA methyltransferase 2
Gene type protein-coding
Also known as MISU, MRT5, SAKI, TRM4
Summary This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Genomic regions Chromosome 5
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NSUN2-201 ENST00000264670.11 3056 767aa Protein coding CCDS3869 Q08J23-1 NM_017755.6 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
NSUN2-206 ENST00000506139.5 2619 732aa Protein coding CCDS54832 Q08J23-2 - TSL:2, GENCODE basic, APPRIS ALT2,
NSUN2-203 ENST00000504374.5 3217 202aa Nonsense mediated decay - A0A140T9Y7 - TSL:2,
NSUN2-209 ENST00000514127.1 614 102aa Nonsense mediated decay - A0A140T9Z1 - CDS 5' incomplete, TSL:3,
NSUN2-204 ENST00000505264.1 472 No protein Processed transcript - - - TSL:4,
NSUN2-205 ENST00000505892.5 3558 No protein Retained intron - - - TSL:1,
NSUN2-208 ENST00000513888.5 1290 No protein Retained intron - - - TSL:2,
NSUN2-207 ENST00000507888.1 715 No protein Retained intron - - - TSL:3,
NSUN2-202 ENST00000502932.1 512 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NSUN2
This KO Strategy loading
Red Cotton™ Notes Gene NSUN2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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