CRISPR-U™ Gene Knockout Cell Line
Strategy
NSUN2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NSUN2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NSUN2 |
| Gene id | 54888 |
| Organism | Homo sapiens |
| Official full symbol | NOP2/Sun RNA methyltransferase 2 |
| Gene type | protein-coding |
| Also known as | MISU, MRT5, SAKI, TRM4 |
| Summary | This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NSUN2-201 | ENST00000264670.11 | 3056 | 767aa | Protein coding | CCDS3869 | Q08J23-1 | NM_017755.6 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| NSUN2-206 | ENST00000506139.5 | 2619 | 732aa | Protein coding | CCDS54832 | Q08J23-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| NSUN2-203 | ENST00000504374.5 | 3217 | 202aa | Nonsense mediated decay | - | A0A140T9Y7 | - | TSL:2, |
| NSUN2-209 | ENST00000514127.1 | 614 | 102aa | Nonsense mediated decay | - | A0A140T9Z1 | - | CDS 5' incomplete, TSL:3, |
| NSUN2-204 | ENST00000505264.1 | 472 | No protein | Processed transcript | - | - | - | TSL:4, |
| NSUN2-205 | ENST00000505892.5 | 3558 | No protein | Retained intron | - | - | - | TSL:1, |
| NSUN2-208 | ENST00000513888.5 | 1290 | No protein | Retained intron | - | - | - | TSL:2, |
| NSUN2-207 | ENST00000507888.1 | 715 | No protein | Retained intron | - | - | - | TSL:3, |
| NSUN2-202 | ENST00000502932.1 | 512 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NSUN2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NSUN2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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