CRISPR-U™ Gene Knockout Cell Line Strategy

GPATCH4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GPATCH4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GPATCH4
Gene id 54865
Organism Homo sapiens
Official full symbol G-patch domain containing 4
Gene type protein-coding
Also known as GPATC4
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GPATCH4-201 ENST00000368232.9 2154 370aa Protein coding CCDS1146 A0A0A0MRK1 NM_182679.3 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
GPATCH4-203 ENST00000438976.6 1159 375aa Protein coding CCDS44245 E9PAV9 - TSL:2, GENCODE basic, APPRIS ALT2,
GPATCH4-202 ENST00000415314.6 934 264aa Protein coding - F8W895 - CDS 3' incomplete, TSL:3,
GPATCH4-204 ENST00000463513.5 857 119aa Nonsense mediated decay - E9PPY4 - TSL:3,
GPATCH4-206 ENST00000474904.5 719 143aa Nonsense mediated decay - F2Z3C6 - TSL:5,
GPATCH4-208 ENST00000497287.5 837 No protein Processed transcript - - - TSL:3,
GPATCH4-215 ENST00000531129.5 532 No protein Processed transcript - - - TSL:5,
GPATCH4-205 ENST00000473910.5 2464 No protein Retained intron - - - TSL:2,
GPATCH4-211 ENST00000506832.6 1029 No protein Retained intron - - - TSL:2,
GPATCH4-213 ENST00000527691.5 781 No protein Retained intron - - - TSL:4,
GPATCH4-207 ENST00000494414.1 740 No protein Retained intron - - - TSL:5,
GPATCH4-216 ENST00000531900.1 653 No protein Retained intron - - - TSL:2,
GPATCH4-210 ENST00000498756.5 634 No protein Retained intron - - - TSL:2,
GPATCH4-214 ENST00000529520.5 552 No protein Retained intron - - - TSL:2,
GPATCH4-209 ENST00000498641.1 534 No protein Retained intron - - - TSL:3,
GPATCH4-212 ENST00000525375.1 513 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GPATCH4
This KO Strategy loading
Red Cotton™ Notes Gene GPATCH4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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