CRISPR-U™ Gene Knockout Cell Line
Strategy
PPID Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPID Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPID |
| Gene id | 5481 |
| Organism | Homo sapiens |
| Official full symbol | peptidylprolyl isomerase D |
| Gene type | protein-coding |
| Also known as | CYP-40, CYPD |
| Summary | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPID-201 | ENST00000307720.4 | 1830 | 370aa | Protein coding | CCDS3801 | E5KN55 Q08752 | NM_005038.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPID-202 | ENST00000507213.1 | 789 | 48aa | Protein coding | - | H0Y8J0 | - | CDS 5' incomplete, TSL:3, |
| PPID-203 | ENST00000512699.1 | 594 | 19aa | Nonsense mediated decay | - | H0Y969 | - | CDS 5' incomplete, TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPID |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPID had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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