CRISPR-U™ Gene Knockout Cell Line Strategy

PPID Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPID Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPID
Gene id 5481
Organism Homo sapiens
Official full symbol peptidylprolyl isomerase D
Gene type protein-coding
Also known as CYP-40, CYPD
Summary The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A.
Genomic regions Chromosome 4
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPID-201 ENST00000307720.4 1830 370aa Protein coding CCDS3801 E5KN55 Q08752 NM_005038.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PPID-202 ENST00000507213.1 789 48aa Protein coding - H0Y8J0 - CDS 5' incomplete, TSL:3,
PPID-203 ENST00000512699.1 594 19aa Nonsense mediated decay - H0Y969 - CDS 5' incomplete, TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPID
This KO Strategy loading
Red Cotton™ Notes Gene PPID had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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