CRISPR-U™ Gene Knockout Cell Line Strategy

SAMD9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human SAMD9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SAMD9
Gene id 54809
Organism Homo sapiens
Official full symbol sterile alpha motif domain containing 9
Gene type protein-coding
Also known as C7orf5, DRIF1, MIRAGE, NFTC, OEF1, OEF2
Summary This gene encodes a sterile alpha motif domain-containing protein. The encoded protein localizes to the cytoplasm and may play a role in regulating cell proliferation and apoptosis. Mutations in this gene are the cause of normophosphatemic familial tumoral calcinosis. Alternate splicing results in multiple transcript variants that encode the same protein.
Genomic regions Chromosome 7
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SAMD9-201 ENST00000379958.3 6806 1589aa Protein coding CCDS34680 Q5K651 NM_017654.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
SAMD9-203 ENST00000620985.4 6754 1589aa Protein coding CCDS34680 Q5K651 - TSL:2, GENCODE basic, APPRIS P1,
SAMD9-202 ENST00000446617.1 3957 1283aa Protein coding - C9JKF1 - CDS 3' incomplete, TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SAMD9
This KO Strategy loading
Red Cotton™ Notes Gene SAMD9 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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