CRISPR-U™ Gene Knockout Cell Line Strategy

PPIB Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPIB Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPIB
Gene id 5479
Organism Homo sapiens
Official full symbol peptidylprolyl isomerase B
Gene type protein-coding
Also known as B, CYP-S1, CYPB, HEL-S-39, OI9, SCYLP
Summary The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.
Genomic regions Chromosome 15
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPIB-201 ENST00000300026.4 893 216aa Protein coding CCDS10191 P23284 NM_000942.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
PPIB-206 ENST00000681397.1 973 216aa Protein coding - - - GENCODE basic, APPRIS ALT1,
PPIB-207 ENST00000681658.1 869 181aa Protein coding - - - GENCODE basic,
PPIB-204 ENST00000680158.1 1050 123aa Nonsense mediated decay - - - -
PPIB-205 ENST00000680343.1 888 No protein Processed transcript - - - -
PPIB-202 ENST00000558492.1 381 No protein Processed transcript - - - TSL:3,
PPIB-203 ENST00000561048.2 4064 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPIB
This KO Strategy loading
Red Cotton™ Notes Gene PPIB had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: