CRISPR-U™ Gene Knockout Cell Line
Strategy
PPARG Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PPARG Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PPARG |
| Gene id | 5468 |
| Organism | Homo sapiens |
| Official full symbol | peroxisome proliferator activated receptor gamma |
| Gene type | protein-coding |
| Also known as | CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma |
| Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 16 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PPARG-201 | ENST00000287820.10 | 1850 | 505aa | Protein coding | CCDS2609 | P37231-1 | - | TSL:1, GENCODE basic, |
| PPARG-208 | ENST00000397026.7 | 2029 | 475aa | Protein coding | - | E9PFV2 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PPARG-205 | ENST00000397010.7 | 2017 | 475aa | Protein coding | - | E9PFV2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PPARG-216 | ENST00000644622.2 | 1962 | 475aa | Protein coding | - | E9PFV2 | - | GENCODE basic, APPRIS P1, |
| PPARG-214 | ENST00000643197.2 | 1894 | 475aa | Protein coding | - | E9PFV2 | - | GENCODE basic, APPRIS P1, |
| PPARG-202 | ENST00000309576.11 | 1870 | 475aa | Protein coding | - | E9PFV2 | - | TSL:2, GENCODE basic, APPRIS P1, |
| PPARG-217 | ENST00000651735.1 | 1774 | 475aa | Protein coding | - | E9PFV2 | NM_138711.6 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PPARG-206 | ENST00000397015.7 | 1772 | 475aa | Protein coding | - | E9PFV2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PPARG-215 | ENST00000643888.2 | 1693 | 475aa | Protein coding | - | E9PFV2 | - | GENCODE basic, APPRIS P1, |
| PPARG-219 | ENST00000652431.1 | 1366 | 371aa | Protein coding | - | A0A494C0T3 | - | GENCODE basic, |
| PPARG-220 | ENST00000652522.1 | 1350 | 376aa | Protein coding | - | A0A494C0D9 | - | CDS 3' incomplete, |
| PPARG-204 | ENST00000397000.6 | 1122 | 248aa | Protein coding | - | E9PFX5 | - | TSL:1, GENCODE basic, |
| PPARG-218 | ENST00000652098.1 | 1058 | 293aa | Protein coding | - | A0A494C012 | - | GENCODE basic, |
| PPARG-209 | ENST00000397029.8 | 686 | 130aa | Protein coding | - | E7EUD1 | - | CDS 3' incomplete, TSL:3, |
| PPARG-210 | ENST00000438682.6 | 560 | 97aa | Protein coding | - | E7EU07 | - | CDS 3' incomplete, TSL:3, |
| PPARG-211 | ENST00000455517.6 | 428 | 41aa | Protein coding | - | Q6L9M1 | - | CDS 3' incomplete, TSL:1, |
| PPARG-207 | ENST00000397023.5 | 2002 | 40aa | Nonsense mediated decay | - | E9PFV3 | - | TSL:5, |
| PPARG-203 | ENST00000396999.3 | 1335 | 184aa | Nonsense mediated decay | - | A0A499FIV4 | - | TSL:1, |
| PPARG-212 | ENST00000477039.5 | 1705 | No protein | Retained intron | - | - | - | TSL:1, |
| PPARG-213 | ENST00000497594.5 | 1600 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PPARG |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PPARG had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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