CRISPR-U™ Gene Knockout Cell Line Strategy

PPARG Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PPARG Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PPARG
Gene id 5468
Organism Homo sapiens
Official full symbol peroxisome proliferator activated receptor gamma
Gene type protein-coding
Also known as CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma
Summary This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
Genomic regions Chromosome 3
Strategy Summary
This gene has 16 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PPARG-201 ENST00000287820.10 1850 505aa Protein coding CCDS2609 P37231-1 - TSL:1, GENCODE basic,
PPARG-208 ENST00000397026.7 2029 475aa Protein coding - E9PFV2 - TSL:5, GENCODE basic, APPRIS P1,
PPARG-205 ENST00000397010.7 2017 475aa Protein coding - E9PFV2 - TSL:1, GENCODE basic, APPRIS P1,
PPARG-216 ENST00000644622.2 1962 475aa Protein coding - E9PFV2 - GENCODE basic, APPRIS P1,
PPARG-214 ENST00000643197.2 1894 475aa Protein coding - E9PFV2 - GENCODE basic, APPRIS P1,
PPARG-202 ENST00000309576.11 1870 475aa Protein coding - E9PFV2 - TSL:2, GENCODE basic, APPRIS P1,
PPARG-217 ENST00000651735.1 1774 475aa Protein coding - E9PFV2 NM_138711.6 GENCODE basic, APPRIS P1, MANE Select v0.92,
PPARG-206 ENST00000397015.7 1772 475aa Protein coding - E9PFV2 - TSL:1, GENCODE basic, APPRIS P1,
PPARG-215 ENST00000643888.2 1693 475aa Protein coding - E9PFV2 - GENCODE basic, APPRIS P1,
PPARG-219 ENST00000652431.1 1366 371aa Protein coding - A0A494C0T3 - GENCODE basic,
PPARG-220 ENST00000652522.1 1350 376aa Protein coding - A0A494C0D9 - CDS 3' incomplete,
PPARG-204 ENST00000397000.6 1122 248aa Protein coding - E9PFX5 - TSL:1, GENCODE basic,
PPARG-218 ENST00000652098.1 1058 293aa Protein coding - A0A494C012 - GENCODE basic,
PPARG-209 ENST00000397029.8 686 130aa Protein coding - E7EUD1 - CDS 3' incomplete, TSL:3,
PPARG-210 ENST00000438682.6 560 97aa Protein coding - E7EU07 - CDS 3' incomplete, TSL:3,
PPARG-211 ENST00000455517.6 428 41aa Protein coding - Q6L9M1 - CDS 3' incomplete, TSL:1,
PPARG-207 ENST00000397023.5 2002 40aa Nonsense mediated decay - E9PFV3 - TSL:5,
PPARG-203 ENST00000396999.3 1335 184aa Nonsense mediated decay - A0A499FIV4 - TSL:1,
PPARG-212 ENST00000477039.5 1705 No protein Retained intron - - - TSL:1,
PPARG-213 ENST00000497594.5 1600 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PPARG
This KO Strategy loading
Red Cotton™ Notes Gene PPARG had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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