CRISPR-U™ Gene Knockout Cell Line
Strategy
PARP14 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PARP14 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PARP14 |
| Gene id | 54625 |
| Organism | Homo sapiens |
| Official full symbol | poly(ADP-ribose) polymerase family member 14 |
| Gene type | protein-coding |
| Also known as | ARTD8, BAL2, PARP-14, pART8 |
| Summary | This gene encodes a member of the poly(ADP-ribose) polymerase (PARP) protein family. The encoded anti-apoptotic protein may regulate aerobic glycolysis and promote survival of cancer cells. Increased expression of this gene has been reported in a variety of tumor types. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PARP14-202 | ENST00000474629.7 | 7694 | 1801aa | Protein coding | CCDS46894 | Q460N5-6 | NM_017554.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PARP14-206 | ENST00000494811.2 | 581 | 185aa | Protein coding | - | H7C4Y3 | - | CDS 3' incomplete, TSL:4, |
| PARP14-201 | ENST00000460683.1 | 8437 | 1521aa | Nonsense mediated decay | - | H7C5R8 | - | CDS 5' incomplete, TSL:5, |
| PARP14-207 | ENST00000649945.1 | 5411 | 292aa | Nonsense mediated decay | - | A0A3B3ITD5 | - | - |
| PARP14-204 | ENST00000475640.1 | 850 | No protein | Processed transcript | - | - | - | TSL:1, |
| PARP14-205 | ENST00000483611.1 | 394 | No protein | Processed transcript | - | - | - | TSL:3, |
| PARP14-203 | ENST00000474669.1 | 5229 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PARP14 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PARP14 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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