CRISPR-U™ Gene Knockout Cell Line Strategy

PARP14 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PARP14 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PARP14
Gene id 54625
Organism Homo sapiens
Official full symbol poly(ADP-ribose) polymerase family member 14
Gene type protein-coding
Also known as ARTD8, BAL2, PARP-14, pART8
Summary This gene encodes a member of the poly(ADP-ribose) polymerase (PARP) protein family. The encoded anti-apoptotic protein may regulate aerobic glycolysis and promote survival of cancer cells. Increased expression of this gene has been reported in a variety of tumor types.
Genomic regions Chromosome 3
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PARP14-202 ENST00000474629.7 7694 1801aa Protein coding CCDS46894 Q460N5-6 NM_017554.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PARP14-206 ENST00000494811.2 581 185aa Protein coding - H7C4Y3 - CDS 3' incomplete, TSL:4,
PARP14-201 ENST00000460683.1 8437 1521aa Nonsense mediated decay - H7C5R8 - CDS 5' incomplete, TSL:5,
PARP14-207 ENST00000649945.1 5411 292aa Nonsense mediated decay - A0A3B3ITD5 - -
PARP14-204 ENST00000475640.1 850 No protein Processed transcript - - - TSL:1,
PARP14-205 ENST00000483611.1 394 No protein Processed transcript - - - TSL:3,
PARP14-203 ENST00000474669.1 5229 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PARP14
This KO Strategy loading
Red Cotton™ Notes Gene PARP14 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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