CRISPR-U™ Gene Knockout Cell Line
Strategy
POU4F2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human POU4F2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | POU4F2 |
| Gene id | 5458 |
| Organism | Homo sapiens |
| Official full symbol | POU class 4 homeobox 2 |
| Gene type | protein-coding |
| Also known as | BRN3.2, BRN3B, Brn-3b |
| Summary | The protein encoded by this gene is a member of the POU-domain transcription factor family and may be involved in maintaining visual system neurons in the retina. The level of the encoded protein is also elevated in a majority of breast cancers, resulting in accelerated tumor growth. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| POU4F2-201 | ENST00000281321.3 | 3144 | 409aa | Protein coding | CCDS34074 | Q12837-1 | NM_004575.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | POU4F2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene POU4F2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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