CRISPR-U™ Gene Knockout Cell Line Strategy

PON2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PON2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PON2
Gene id 5445
Organism Homo sapiens
Official full symbol paraoxonase 2
Gene type protein-coding
Summary This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.
Genomic regions Chromosome 7
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PON2-202 ENST00000433091.6 1726 342aa Protein coding CCDS47644 Q15165-3 - TSL:1, GENCODE basic,
PON2-218 ENST00000633531.1 1653 354aa Protein coding CCDS5640 Q15165-2 - TSL:1, GENCODE basic, APPRIS P1,
PON2-201 ENST00000222572.8 1610 354aa Protein coding CCDS5640 Q15165-2 NM_000305.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PON2-217 ENST00000633192.1 1876 375aa Protein coding - A0A0J9YXF2 - TSL:1, GENCODE basic,
PON2-212 ENST00000490778.5 694 69aa Protein coding - A0A0J9YYF3 - CDS 3' incomplete, TSL:5,
PON2-208 ENST00000469926.5 645 113aa Protein coding - A0A0J9YXU7 - CDS 3' incomplete, TSL:3,
PON2-210 ENST00000478801.5 555 32aa Protein coding - A0A0J9YVW3 - CDS 3' incomplete, TSL:4,
PON2-214 ENST00000493290.5 550 53aa Protein coding - A0A0J9YYF0 - CDS 3' incomplete, TSL:4,
PON2-204 ENST00000455123.5 1515 219aa Nonsense mediated decay - G3XAK4 - TSL:1,
PON2-203 ENST00000446142.5 1099 50aa Nonsense mediated decay - A0A2Q2SAK4 - TSL:1,
PON2-211 ENST00000483292.5 699 122aa Nonsense mediated decay - A0A0J9YYJ1 - CDS 5' incomplete, TSL:3,
PON2-216 ENST00000632034.1 570 84aa Nonsense mediated decay - A0A0J9YXM1 - TSL:2,
PON2-213 ENST00000491069.5 566 84aa Nonsense mediated decay - A0A0J9YXM1 - TSL:2,
PON2-206 ENST00000460873.5 569 No protein Processed transcript - - - TSL:4,
PON2-209 ENST00000471883.1 451 No protein Processed transcript - - - TSL:3,
PON2-205 ENST00000459842.1 627 No protein Retained intron - - - TSL:2,
PON2-215 ENST00000493469.5 579 No protein Retained intron - - - TSL:2,
PON2-207 ENST00000469716.1 573 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PON2
This KO Strategy loading
Red Cotton™ Notes Gene PON2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

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