CRISPR-U™ Gene Knockout Cell Line
Strategy
PON2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PON2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PON2 |
| Gene id | 5445 |
| Organism | Homo sapiens |
| Official full symbol | paraoxonase 2 |
| Gene type | protein-coding |
| Summary | This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PON2-202 | ENST00000433091.6 | 1726 | 342aa | Protein coding | CCDS47644 | Q15165-3 | - | TSL:1, GENCODE basic, |
| PON2-218 | ENST00000633531.1 | 1653 | 354aa | Protein coding | CCDS5640 | Q15165-2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PON2-201 | ENST00000222572.8 | 1610 | 354aa | Protein coding | CCDS5640 | Q15165-2 | NM_000305.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PON2-217 | ENST00000633192.1 | 1876 | 375aa | Protein coding | - | A0A0J9YXF2 | - | TSL:1, GENCODE basic, |
| PON2-212 | ENST00000490778.5 | 694 | 69aa | Protein coding | - | A0A0J9YYF3 | - | CDS 3' incomplete, TSL:5, |
| PON2-208 | ENST00000469926.5 | 645 | 113aa | Protein coding | - | A0A0J9YXU7 | - | CDS 3' incomplete, TSL:3, |
| PON2-210 | ENST00000478801.5 | 555 | 32aa | Protein coding | - | A0A0J9YVW3 | - | CDS 3' incomplete, TSL:4, |
| PON2-214 | ENST00000493290.5 | 550 | 53aa | Protein coding | - | A0A0J9YYF0 | - | CDS 3' incomplete, TSL:4, |
| PON2-204 | ENST00000455123.5 | 1515 | 219aa | Nonsense mediated decay | - | G3XAK4 | - | TSL:1, |
| PON2-203 | ENST00000446142.5 | 1099 | 50aa | Nonsense mediated decay | - | A0A2Q2SAK4 | - | TSL:1, |
| PON2-211 | ENST00000483292.5 | 699 | 122aa | Nonsense mediated decay | - | A0A0J9YYJ1 | - | CDS 5' incomplete, TSL:3, |
| PON2-216 | ENST00000632034.1 | 570 | 84aa | Nonsense mediated decay | - | A0A0J9YXM1 | - | TSL:2, |
| PON2-213 | ENST00000491069.5 | 566 | 84aa | Nonsense mediated decay | - | A0A0J9YXM1 | - | TSL:2, |
| PON2-206 | ENST00000460873.5 | 569 | No protein | Processed transcript | - | - | - | TSL:4, |
| PON2-209 | ENST00000471883.1 | 451 | No protein | Processed transcript | - | - | - | TSL:3, |
| PON2-205 | ENST00000459842.1 | 627 | No protein | Retained intron | - | - | - | TSL:2, |
| PON2-215 | ENST00000493469.5 | 579 | No protein | Retained intron | - | - | - | TSL:2, |
| PON2-207 | ENST00000469716.1 | 573 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PON2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PON2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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