CRISPR-U™ Gene Knockout Cell Line Strategy

DNAJC10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DNAJC10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DNAJC10
Gene id 54431
Organism Homo sapiens
Official full symbol DnaJ heat shock protein family (Hsp40) member C10
Gene type protein-coding
Also known as ERdj5, JPDI, MTHr, PDIA19
Summary This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DNAJC10-201 ENST00000264065.12 20144 793aa Protein coding CCDS33345 Q8IXB1-1 NM_018981.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DNAJC10-209 ENST00000616986.5 20006 747aa Protein coding CCDS74613 Q8IXB1-2 - TSL:1, GENCODE basic,
DNAJC10-216 ENST00000680258.1 20086 773aa Protein coding - - - GENCODE basic,
DNAJC10-214 ENST00000679884.1 19812 768aa Protein coding - - - GENCODE basic,
DNAJC10-220 ENST00000680667.1 19645 626aa Protein coding - - - GENCODE basic,
DNAJC10-208 ENST00000537515.5 1669 332aa Protein coding - Q8IXB1-3 - TSL:1, GENCODE basic,
DNAJC10-222 ENST00000681139.1 21051 183aa Nonsense mediated decay - - - -
DNAJC10-217 ENST00000680480.1 21035 332aa Nonsense mediated decay - - - -
DNAJC10-224 ENST00000681873.1 20984 272aa Nonsense mediated decay - - - -
DNAJC10-211 ENST00000650903.2 20933 301aa Nonsense mediated decay - A0A494C1G5 - -
DNAJC10-223 ENST00000681146.1 20757 286aa Nonsense mediated decay - - - -
DNAJC10-218 ENST00000680484.1 20685 259aa Nonsense mediated decay - - - -
DNAJC10-212 ENST00000679491.1 19989 247aa Nonsense mediated decay - - - -
DNAJC10-213 ENST00000679526.1 19989 247aa Nonsense mediated decay - - - -
DNAJC10-202 ENST00000418559.6 19607 275aa Nonsense mediated decay - E7EP04 - TSL:1,
DNAJC10-203 ENST00000444005.1 605 121aa Nonsense mediated decay - H7C1G0 - CDS 5' incomplete, TSL:4,
DNAJC10-205 ENST00000469118.5 545 No protein Processed transcript - - - TSL:5,
DNAJC10-206 ENST00000491074.6 20962 No protein Retained intron - - - TSL:1,
DNAJC10-215 ENST00000680060.1 20345 No protein Retained intron - - - -
DNAJC10-221 ENST00000681122.1 18913 No protein Retained intron - - - -
DNAJC10-210 ENST00000640034.2 2306 No protein Retained intron - - - TSL:5,
DNAJC10-219 ENST00000680648.1 2050 No protein Retained intron - - - -
DNAJC10-207 ENST00000494462.1 673 No protein Retained intron - - - TSL:3,
DNAJC10-204 ENST00000459930.1 424 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DNAJC10
This KO Strategy loading
Red Cotton™ Notes Gene DNAJC10 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: