CRISPR-U™ Gene Knockout Cell Line Strategy

POLB Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human POLB Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol POLB
Gene id 5423
Organism Homo sapiens
Official full symbol DNA polymerase beta
Gene type protein-coding
Summary The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
Genomic regions Chromosome 8
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
POLB-201 ENST00000265421.9 1290 335aa Protein coding CCDS6129 P06746 NM_002690.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
POLB-204 ENST00000518925.5 983 280aa Protein coding - E7EW18 - CDS 3' incomplete, TSL:5,
POLB-209 ENST00000521290.5 863 237aa Protein coding - H0YBJ0 - CDS 5' incomplete, TSL:5,
POLB-211 ENST00000521492.1 786 54aa Protein coding - E5RHZ4 - TSL:2, GENCODE basic,
POLB-203 ENST00000518579.5 744 239aa Protein coding - H0YBX1 - CDS 5' incomplete, TSL:5,
POLB-208 ENST00000520008.5 725 51aa Protein coding - E5RJ55 - CDS 3' incomplete, TSL:2,
POLB-217 ENST00000532157.5 675 38aa Protein coding - E9PIC6 - CDS 3' incomplete, TSL:3,
POLB-202 ENST00000517393.1 401 106aa Protein coding - H0YAV8 - CDS 5' incomplete, TSL:3,
POLB-213 ENST00000522610.5 1341 193aa Nonsense mediated decay - E5RIJ0 - TSL:5,
POLB-207 ENST00000519771.5 720 42aa Nonsense mediated decay - E5RG65 - TSL:5,
POLB-215 ENST00000524208.5 653 29aa Nonsense mediated decay - H0YB29 - CDS 5' incomplete, TSL:5,
POLB-216 ENST00000530566.1 590 No protein Processed transcript - - - TSL:4,
POLB-206 ENST00000519524.1 655 No protein Retained intron - - - TSL:5,
POLB-214 ENST00000523465.1 649 No protein Retained intron - - - TSL:3,
POLB-210 ENST00000521418.1 628 No protein Retained intron - - - TSL:2,
POLB-212 ENST00000522297.1 591 No protein Retained intron - - - TSL:2,
POLB-205 ENST00000519094.5 561 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene POLB
This KO Strategy loading
Red Cotton™ Notes Gene POLB had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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