CRISPR-U™ Gene Knockout Cell Line
Strategy
POLB Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human POLB Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | POLB |
| Gene id | 5423 |
| Organism | Homo sapiens |
| Official full symbol | DNA polymerase beta |
| Gene type | protein-coding |
| Summary | The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| POLB-201 | ENST00000265421.9 | 1290 | 335aa | Protein coding | CCDS6129 | P06746 | NM_002690.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| POLB-204 | ENST00000518925.5 | 983 | 280aa | Protein coding | - | E7EW18 | - | CDS 3' incomplete, TSL:5, |
| POLB-209 | ENST00000521290.5 | 863 | 237aa | Protein coding | - | H0YBJ0 | - | CDS 5' incomplete, TSL:5, |
| POLB-211 | ENST00000521492.1 | 786 | 54aa | Protein coding | - | E5RHZ4 | - | TSL:2, GENCODE basic, |
| POLB-203 | ENST00000518579.5 | 744 | 239aa | Protein coding | - | H0YBX1 | - | CDS 5' incomplete, TSL:5, |
| POLB-208 | ENST00000520008.5 | 725 | 51aa | Protein coding | - | E5RJ55 | - | CDS 3' incomplete, TSL:2, |
| POLB-217 | ENST00000532157.5 | 675 | 38aa | Protein coding | - | E9PIC6 | - | CDS 3' incomplete, TSL:3, |
| POLB-202 | ENST00000517393.1 | 401 | 106aa | Protein coding | - | H0YAV8 | - | CDS 5' incomplete, TSL:3, |
| POLB-213 | ENST00000522610.5 | 1341 | 193aa | Nonsense mediated decay | - | E5RIJ0 | - | TSL:5, |
| POLB-207 | ENST00000519771.5 | 720 | 42aa | Nonsense mediated decay | - | E5RG65 | - | TSL:5, |
| POLB-215 | ENST00000524208.5 | 653 | 29aa | Nonsense mediated decay | - | H0YB29 | - | CDS 5' incomplete, TSL:5, |
| POLB-216 | ENST00000530566.1 | 590 | No protein | Processed transcript | - | - | - | TSL:4, |
| POLB-206 | ENST00000519524.1 | 655 | No protein | Retained intron | - | - | - | TSL:5, |
| POLB-214 | ENST00000523465.1 | 649 | No protein | Retained intron | - | - | - | TSL:3, |
| POLB-210 | ENST00000521418.1 | 628 | No protein | Retained intron | - | - | - | TSL:2, |
| POLB-212 | ENST00000522297.1 | 591 | No protein | Retained intron | - | - | - | TSL:2, |
| POLB-205 | ENST00000519094.5 | 561 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | POLB |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene POLB had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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