CRISPR-U™ Gene Knockout Cell Line Strategy

PODXL Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PODXL Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PODXL
Gene id 5420
Organism Homo sapiens
Official full symbol podocalyxin like
Gene type protein-coding
Also known as Gp200, PC, PCLP, PCLP-1
Summary This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.
Genomic regions Chromosome 7
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PODXL-202 ENST00000378555.8 5986 558aa Protein coding CCDS34755 O00592-1 NM_001018111.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
PODXL-201 ENST00000322985.9 2011 526aa Protein coding CCDS47714 O00592-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PODXL-203 ENST00000446198.5 6170 324aa Nonsense mediated decay - G3V0E6 - TSL:2,
PODXL-204 ENST00000465001.1 530 No protein Processed transcript - - - TSL:4,
PODXL-207 ENST00000487965.1 809 No protein Retained intron - - - TSL:2,
PODXL-206 ENST00000484346.1 797 No protein Retained intron - - - TSL:2,
PODXL-205 ENST00000482581.1 588 No protein Retained intron - - - TSL:2,
PODXL-208 ENST00000490761.5 571 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PODXL
This KO Strategy loading
Red Cotton™ Notes Gene PODXL had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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