CRISPR-U™ Gene Knockout Cell Line
Strategy
PODXL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PODXL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PODXL |
| Gene id | 5420 |
| Organism | Homo sapiens |
| Official full symbol | podocalyxin like |
| Gene type | protein-coding |
| Also known as | Gp200, PC, PCLP, PCLP-1 |
| Summary | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PODXL-202 | ENST00000378555.8 | 5986 | 558aa | Protein coding | CCDS34755 | O00592-1 | NM_001018111.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| PODXL-201 | ENST00000322985.9 | 2011 | 526aa | Protein coding | CCDS47714 | O00592-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PODXL-203 | ENST00000446198.5 | 6170 | 324aa | Nonsense mediated decay | - | G3V0E6 | - | TSL:2, |
| PODXL-204 | ENST00000465001.1 | 530 | No protein | Processed transcript | - | - | - | TSL:4, |
| PODXL-207 | ENST00000487965.1 | 809 | No protein | Retained intron | - | - | - | TSL:2, |
| PODXL-206 | ENST00000484346.1 | 797 | No protein | Retained intron | - | - | - | TSL:2, |
| PODXL-205 | ENST00000482581.1 | 588 | No protein | Retained intron | - | - | - | TSL:2, |
| PODXL-208 | ENST00000490761.5 | 571 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PODXL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PODXL had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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