CRISPR-U™ Gene Knockout Cell Line
Strategy
POLE3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human POLE3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | POLE3 |
| Gene id | 54107 |
| Organism | Homo sapiens |
| Official full symbol | DNA polymerase epsilon 3, accessory subunit |
| Gene type | protein-coding |
| Also known as | CHARAC17, CHRAC17, CHRAC2, YBL1, p17 |
| Summary | POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| POLE3-202 | ENST00000374171.5 | 2194 | 147aa | Protein coding | CCDS6795 | A0A024R829 Q9NRF9 | NM_017443.5 | TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| POLE3-201 | ENST00000374169.7 | 2109 | 147aa | Protein coding | CCDS6795 | A0A024R829 Q9NRF9 | - | TSL:1, GENCODE basic, APPRIS P1, |
| POLE3-204 | ENST00000479871.1 | 2396 | No protein | Processed transcript | - | - | - | TSL:2, |
| POLE3-203 | ENST00000475080.1 | 455 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | POLE3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene POLE3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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