CRISPR-U™ Gene Knockout Cell Line Strategy

POLE3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human POLE3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol POLE3
Gene id 54107
Organism Homo sapiens
Official full symbol DNA polymerase epsilon 3, accessory subunit
Gene type protein-coding
Also known as CHARAC17, CHRAC17, CHRAC2, YBL1, p17
Summary POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
Genomic regions Chromosome 9
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
POLE3-202 ENST00000374171.5 2194 147aa Protein coding CCDS6795 A0A024R829 Q9NRF9 NM_017443.5 TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92,
POLE3-201 ENST00000374169.7 2109 147aa Protein coding CCDS6795 A0A024R829 Q9NRF9 - TSL:1, GENCODE basic, APPRIS P1,
POLE3-204 ENST00000479871.1 2396 No protein Processed transcript - - - TSL:2,
POLE3-203 ENST00000475080.1 455 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene POLE3
This KO Strategy loading
Red Cotton™ Notes Gene POLE3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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